Ben Abdallah F, Chaieb K, Snoussi M, Bakhrouf A, Gaddour K
Curr Microbiol. 2007 Dec;55(6):485-91. doi: 10.1007/s00284-007-9017-4. Epub 2007 Sep 9.
In seawater, enteric bacteria evolve toward a stressed state that is difficult to identify because of major alterations of their phenotype. In this study, we incubated four reference strains of S. enterica serovar Typhimurium in seawater microcosms for 10 months and studied the modifications of their main phenotypic characters. All of the strains lost some key characters used for traditional identification of the Salmonella genus. They became able to produce acetoin, and tryptophane deaminase activity became positive, but they lost the capacity to use rhamnose. We were able to show some modifications of the level of enzymatic profile as well as in their antibiotic susceptibility. The atypical cells of S. enterica serovar Typhimurium were identified by polymerase chain reaction (PCR) methods using the internal transcribed spacer region, and they were confirmed by multiplex PCR after the simultaneous amplification of the phoP, Hin, and H-li genes.
在海水中，肠道细菌会朝着一种应激状态演变，由于其表型发生重大改变，这种状态难以识别。在本研究中，我们将四株肠炎沙门氏菌血清型鼠伤寒沙门氏菌参考菌株在海水微观世界中培养10个月，并研究其主要表型特征的变化。所有菌株都失去了一些用于传统鉴定沙门氏菌属的关键特征。它们变得能够产生3-羟基丁酮，色氨酸脱氨酶活性变为阳性，但失去了利用鼠李糖的能力。我们能够展示酶谱水平以及抗生素敏感性方面的一些变化。肠炎沙门氏菌血清型鼠伤寒沙门氏菌的非典型细胞通过使用内部转录间隔区的聚合酶链反应（PCR）方法进行鉴定，并在同时扩增phoP、Hin和H-li基因后通过多重PCR得到确认。
