用于检测和定量分析抗人类免疫缺陷病毒抗体的改进免疫印迹法。
Improved immunoblotting for the detection and quantitative analysis of antibodies to human immunodeficiency virus.
作者信息
Portincasa P, Conti G, Gerloni M, Chezzi C
机构信息
Institute of Microbiology, University of Parma, Medical School, Italy.
出版信息
Eur J Epidemiol. 1991 Nov;7(6):705-8. doi: 10.1007/BF00218688.
PMID:1783068
Abstract
Detection of antibodies to single HIV-1 proteins was performed by modified Western-blotting procedure. The use of [35S]-Streptavidin instead of Avidin-peroxidase conjugate greatly enhances the sensitivity of the method and enables useful quantitative analysis of results.
摘要
采用改良的蛋白质印迹法检测针对单一HIV-1蛋白的抗体。使用[35S] -链霉亲和素替代抗生物素蛋白-过氧化物酶结合物，极大地提高了该方法的灵敏度，并能够对结果进行有效的定量分析。
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