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用于检测和定量分析抗人类免疫缺陷病毒抗体的改进免疫印迹法。

Improved immunoblotting for the detection and quantitative analysis of antibodies to human immunodeficiency virus.

作者信息

Portincasa P, Conti G, Gerloni M, Chezzi C

机构信息

Institute of Microbiology, University of Parma, Medical School, Italy.

出版信息

Eur J Epidemiol. 1991 Nov;7(6):705-8. doi: 10.1007/BF00218688.

DOI:10.1007/BF00218688
PMID:1783068
Abstract

Detection of antibodies to single HIV-1 proteins was performed by modified Western-blotting procedure. The use of [35S]-Streptavidin instead of Avidin-peroxidase conjugate greatly enhances the sensitivity of the method and enables useful quantitative analysis of results.

摘要

采用改良的蛋白质印迹法检测针对单一HIV-1蛋白的抗体。使用[35S] -链霉亲和素替代抗生物素蛋白-过氧化物酶结合物,极大地提高了该方法的灵敏度,并能够对结果进行有效的定量分析。

相似文献

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2
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本文引用的文献

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HLA antibodies in blood donors with reactive screening tests for antibody to the immunodeficiency virus.免疫缺陷病毒抗体筛查试验呈反应性的献血者中的人类白细胞抗原抗体
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Low sensitivity of ELISA testing in early HIV infection.酶联免疫吸附测定(ELISA)检测在早期HIV感染中的低敏感性。
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Practical diagnostic testing for human immunodeficiency virus.人类免疫缺陷病毒的实用诊断检测
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HLA antibodies as a cause of false-positive reactions in screening enzyme immunoassays for antibodies to human T-lymphotropic virus type III.
Transfusion. 1986 Jan-Feb;26(1):113-5. doi: 10.1046/j.1537-2995.1986.26186124012.x.
5
Sensitivity of western blotting (compared with ELISA and immunofluorescence) during seroconversion after HTLV-III infection.人类嗜T淋巴细胞病毒III型(HTLV-III)感染后血清转化期间蛋白质印迹法(与酶联免疫吸附测定法和免疫荧光法相比)的敏感性。
Lancet. 1986 May 17;1(8490):1151-2. doi: 10.1016/s0140-6736(86)91862-3.
6
HLA antibodies detected by ELISA HTLV-III antibody kits.通过ELISA HTLV-III抗体试剂盒检测到的HLA抗体。
Lancet. 1985 Aug 17;2(8451):397. doi: 10.1016/s0140-6736(85)92544-9.
7
HLA-DR antibodies and HTLV-III antibody ELISA testing.人类白细胞抗原-DR抗体和人类嗜T淋巴细胞病毒III型抗体酶联免疫吸附测定
Lancet. 1985 Jul 20;2(8447):157. doi: 10.1016/s0140-6736(85)90261-2.
8
HLA DR4 antibodies cause positive HTLV-III antibody ELISA results.人类白细胞抗原DR4抗体导致人类嗜T淋巴细胞病毒III型抗体酶联免疫吸附测定结果呈阳性。
Lancet. 1985 May 25;1(8439):1222-3. doi: 10.1016/s0140-6736(85)92910-1.