Byamungu N, Darras V M, Kühn E R
Laboratory of Comparative Endocrinology, Catholic University of Leuven, Belgium.
Gen Comp Endocrinol. 1991 Nov;84(2):183-91. doi: 10.1016/0016-6480(91)90041-4.
An immunoadsorbent matrix using antibodies against porcine follicle-stimulating hormone (pFSH), a high heterothyrotropic stimulant in tilapia, was used to purify tilapia thyrotropic hormone (t-TSH) from crude pituitary extracts. A homologous bioassay monitored TSH bioactivity during the purification. Thyroid hormones (thyroxine, T4; triiodothyronine, T3; and reverse triiodothyronine, rT3) and testosterone were measured in vivo in Tilapia nilotica. TSH activity eluted as one major peak at pH 2.8 using a PBS-glycine buffer. The TSH fraction increased plasma T4 and plasma rT3. The potency of tTSH was comparable to that of pituitary extract or its Con A II fraction; however, pFSH was a stronger thyroid stimulant. tTSH had no effect on plasma T3 levels and was free of gonadotropic activity, as indicated by its failure to alter plasma testosterone concentrations. Chromatographic and electrophoretic analyses demonstrated a high degree of purity. Like other vertebrate TSHs, the tTSH appeared to have a subunit structure with a possible microgeneity in one subunit.
使用针对罗非鱼中高促甲状腺活性刺激物猪促卵泡激素（pFSH）的抗体的免疫吸附基质，从垂体粗提物中纯化罗非鱼促甲状腺激素（t-TSH）。在纯化过程中，通过同源生物测定法监测TSH的生物活性。在尼罗罗非鱼体内测量甲状腺激素（甲状腺素，T4；三碘甲状腺原氨酸，T3；和反三碘甲状腺原氨酸，rT3）以及睾酮。使用PBS-甘氨酸缓冲液时，TSH活性在pH 2.8处洗脱为一个主要峰。TSH组分可提高血浆T4和血浆rT3水平。tTSH的效力与垂体提取物或其伴刀豆球蛋白A II组分相当；然而，pFSH是更强的甲状腺刺激物。tTSH对血浆T3水平无影响，且无促性腺活性，这表现为它未能改变血浆睾酮浓度。色谱和电泳分析表明其纯度很高。与其他脊椎动物的TSH一样，tTSH似乎具有亚基结构，其中一个亚基可能存在微小异质性。
