牛蛙（北美牛蛙）幼体变态过程中垂体中糖蛋白激素（促黄体生成素、促卵泡生成素和促甲状腺激素）亚基的差异表现。

Differential appearance of the subunits of glycoprotein hormones (LH, FSH, and TSH) in the pituitary of bullfrog (Rana catesbeiana) larvae during metamorphosis.

作者信息

Tanaka S, Sakai M, Park M K, Kurosumi K

机构信息

Department of Morphology, Gunma University, Maebashi, Japan.

出版信息

Gen Comp Endocrinol. 1991 Nov;84(2):318-27. doi: 10.1016/0016-6480(91)90055-b.

DOI:10.1016/0016-6480(91)90055-b

PMID:1783276

Abstract

The ontogeny of gonadotrophs and thyrotrophs in the bullfrog pituitary was examined immunohistochemically using monoclonal antibodies against bullfrog lutropin beta-subunit (LH beta), follitropin beta-subunit (FSH beta) and its alpha-subunit, and polyclonal anti-human thyrotropin beta-subunit (TSH beta) serum. Immunoreactive alpha-subunit- and TSH beta-, but not FSH beta- and LH beta-containing cells were observed at embryonic stage 24 (Shumway's classification). Immunoreactive FSH beta cells first appeared at Taylor-Korllos stage V, and immunoreactive LH beta cells at stage X. Throughout metamorphosis, several gonadotrophs containing both FSH and LH were found in the ventrocaudal region, but most gonadotrophs contained only FSH. Immunoreactive alpha-subunit cells were always more frequent than the sum of immunoreactive beta-subunit cells, which was confirmed by quantitative studies using immunohistochemical and RIA techniques.

摘要

利用抗牛蛙促黄体生成素β亚基（LHβ）、促卵泡生成素β亚基（FSHβ）及其α亚基的单克隆抗体，以及抗人促甲状腺素β亚基（TSHβ）多克隆血清，通过免疫组织化学方法研究了牛蛙垂体中促性腺激素细胞和促甲状腺激素细胞的个体发生。在胚胎第24期（Shumway分类）观察到免疫反应性α亚基和TSHβ亚基阳性细胞，但未观察到FSHβ亚基和LHβ亚基阳性细胞。免疫反应性FSHβ细胞首次出现在Taylor-Korllos第V期，免疫反应性LHβ细胞出现在第X期。在整个变态过程中，在腹尾区域发现了几种同时含有FSH和LH的促性腺激素细胞，但大多数促性腺激素细胞只含有FSH。免疫反应性α亚基阳性细胞总是比免疫反应性β亚基阳性细胞的总和更常见，这通过免疫组织化学和放射免疫分析技术的定量研究得到了证实。