用于检测动物饲料中磺胺二甲嘧啶污染的竞争性直接酶联免疫吸附筛选测定法。

Competitive direct enzyme-linked immunosorbent screening assay for the detection of sulfamethazine contamination of animal feeds.

作者信息

Dixon-Holland D E, Katz S E

机构信息

Rutgers University, Cook College, Department of Biochemistry and Microbiology, New Brunswick, NJ 08903-0231.

出版信息

J Assoc Off Anal Chem. 1991 Sep-Oct;74(5):784-9.

PMID:1783586

Abstract

A sensitive screening method has been developed for detecting sulfamethazine (SMZ) contamination of feeds by using either polyclonal or monoclonal antibodies and a direct competitive enzyme-linked immunosorbent screening assay (ELISA). Feed samples of 25.0 g are extracted with 0.5N HCl and centrifuged. The extract is adjusted to pH 7.0 with 3.0N NaOH and recentrifuged. This pH-adjusted extract is used in the ELISA. Levels as low as 0.004 micrograms SMZ/g feed were detected in supplemented extracts by polyclonal antibodies; levels of 0.4 micrograms SMZ/g feed were detected by a monoclonal antibody.

摘要

已开发出一种灵敏的筛选方法，通过使用多克隆抗体或单克隆抗体以及直接竞争酶联免疫吸附筛选测定法（ELISA）来检测饲料中的磺胺二甲嘧啶（SMZ）污染。取25.0克饲料样品，用0.5N盐酸提取并离心。提取物用3.0N氢氧化钠调至pH 7.0，然后再次离心。这种pH调节后的提取物用于ELISA。多克隆抗体在添加提取物中检测到低至0.004微克SMZ/克饲料的水平；单克隆抗体检测到的水平为0.4微克SMZ/克饲料。