伴刀豆球蛋白A刺激可改变兔胸腺细胞质膜的脂质和蛋白质结构。一项激光拉曼研究。
Concanavalin A stimulation modifies the lipid and protein structure or rabbit thymocyte plasma membranes. A laser raman study.
作者信息
Schmidt-Ullrich R, Verma S P, Wallach D F
出版信息
Biochim Biophys Acta. 1976 Mar 19;426(3):477-88. doi: 10.1016/0005-2736(76)90392-8.
PMID:178364
Abstract
(1) We have compared the laser Raman spectra of isolated plasma membranes from resting rabbit thymocytes and cells mitogenically stimulated with concanavalin A. (2) Major alterations in the CH stretching, CH deformation and CC stretching regions indicate a different lipid architecture in the membranes from activated cells. (3) Spectral changes in the Amide I and II regions, by reference to the spectra of model compounds indicate greater protein amidation in the membranes from stimulated cells.
摘要
(1) 我们比较了来自静息兔胸腺细胞以及用伴刀豆球蛋白A进行有丝分裂刺激的细胞的分离质膜的激光拉曼光谱。(2) CH伸缩、CH变形和CC伸缩区域的主要变化表明活化细胞的膜中脂质结构不同。(3) 通过参考模型化合物的光谱，酰胺I和II区域的光谱变化表明受刺激细胞的膜中蛋白质酰胺化程度更高。
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