Nijjar M S, Grimmelt B, Brown J
Department of Anatomy and Physiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.
J Chromatogr. 1991 Aug 23;568(2):393-406. doi: 10.1016/0378-4347(91)80177-e.
Domoic acid was the primary neurotoxin in blue mussel (Mytilus edulis) that caused poisoning in humans. Further research showed that the algae, Nitzschia pungens, was the source of this toxin. In this study, a method for the extraction and purification of domoic acid from contaminated mussels and phytoplankton was developed. Domoic acid was extracted from these sources by treatment with a mixture of chloroform and methanol (1:2, v/v). The resulting extract was subjected to ultrafiltration through a PM1 Millipore filter, followed by repeated high-performance liquid chromatography on a reversed-phase column. The purity and yield of domoic acid prepared by this method are compared with two previously described methods of extraction. The current method is relatively simple, rapid, and results in improved recovery with comparable purity of domoic acid.
软骨藻酸是蓝贻贝（紫贻贝）中导致人类中毒的主要神经毒素。进一步研究表明，尖刺菱形藻是这种毒素的来源。在本研究中，开发了一种从受污染的贻贝和浮游植物中提取和纯化软骨藻酸的方法。通过用氯仿和甲醇的混合物（1:2，v/v）处理从这些来源中提取软骨藻酸。所得提取物通过PM1密理博过滤器进行超滤，然后在反相柱上反复进行高效液相色谱。将通过该方法制备的软骨藻酸的纯度和产率与先前描述的两种提取方法进行比较。目前的方法相对简单、快速，并且在软骨藻酸纯度相当的情况下回收率有所提高。
