采用高效液相色谱法测定眼中的三氟胸苷。

Determination of trifluorothymidine in the eye using high-performance liquid chromatography.

作者信息

Riegel M R, Ellis P P

机构信息

Department of Ophthalmology, University of Colorado, School of Medicine, Denver 80262.

出版信息

J Chromatogr. 1991 Aug 23;568(2):467-74. doi: 10.1016/0378-4347(91)80185-f.

DOI:10.1016/0378-4347(91)80185-f

PMID:1783652

Abstract

A high-performance liquid chromatographic assay for trifluorothymidine in biological matrices of the eye is presented. Sample pretreatment was based on liquid-liquid extraction with ethyl acetate. Following evaporation, the residue was analyzed using a reversed-phase octadecylsilane column with ultraviolet detection. The lower limit of detection is ca. 50 ng/ml (ca. 3 ng on column). The method is simple, reproducible and selective. Concentrations of trifluorothymidine in the aqueous humour, vitreous humour and cornea of normal rabbits after a 50-microliters topical dose of 1% trifluorothymidine are reported.

摘要

本文介绍了一种用于测定眼生物基质中三氟胸苷的高效液相色谱法。样品预处理基于用乙酸乙酯进行液-液萃取。蒸发后，使用带有紫外检测的反相十八烷基硅烷柱对残留物进行分析。检测下限约为50 ng/ml（柱上约3 ng）。该方法简单、可重复且具有选择性。报告了正常兔经局部给予50微升1%三氟胸苷后房水、玻璃体液和角膜中三氟胸苷的浓度。