Del Nozal M J, Bernal J L, Pampliega A, Marinero P, López M I, Coco R
Department of Analytical Chemistry, University de Valladolid, Spain.
J Chromatogr A. 1996 Mar 15;727(2):231-8. doi: 10.1016/0021-9673(95)01081-5.
A high-performance liquid chromatographic method for the determination of vancomycin in rabbit serum, vitreous and aqueous humour has been developed. No clean-up step was necessary for vitreous and aqueous humour samples. For serum samples liquid-liquid and solid-phase extraction were tested and the best results were achieved using C18 cartridges. The extracts were analyzed on a C18 reversed-phase column, using a mixture of 0.05 M phosphate buffer (pH 4) with 10% of acetonitrile as mobile phase. The detection was carried out at 198 nm, which allows higher sensitivity. The average quantitation limit obtained was 0.03 micrograms/ml. The method has been applied to the study of the residual quantities of vancomycin in serum and rabbit eyes after intravitreal administration of the drug in endophthalmitis treatment.
已开发出一种用于测定兔血清、玻璃体和房水中万古霉素的高效液相色谱法。玻璃体和房水样本无需净化步骤。对于血清样本，测试了液-液萃取和固相萃取，使用C18柱获得了最佳结果。提取物在C18反相柱上进行分析，使用0.05 M磷酸盐缓冲液（pH 4）与10%乙腈的混合物作为流动相。检测在198 nm处进行，可实现更高的灵敏度。获得的平均定量限为0.03微克/毫升。该方法已应用于眼内炎治疗中玻璃体腔内注射药物后兔血清和眼中万古霉素残留量的研究。
