高效液相色谱法测定鸡肝中游离棉酚

High-performance liquid chromatographic method for the determination of free gossypol in chicken liver.

作者信息

Botsoglou N A

机构信息

Laboratory of Nutrition, School of Veterinary Medicine, Aristotelian University, Thessaloniki, Greece.

出版信息

J Chromatogr. 1991 Dec 20;587(2):333-7. doi: 10.1016/0021-9673(91)85179-j.

DOI:10.1016/0021-9673(91)85179-j

PMID:1783672

Abstract

A high-performance liquid chromatographic method for the determination of free gossypol in chicken liver at levels down to 0.5 ppm has been developed. Tissue was deproteinized with acetonitrile in presence of ascorbic acid and the filtrate was subjected to hydrolysis with hydrochloric acid. The liberated pure gossypol was partitioned into chloroform and analysed by gradient elution on a 10-microns C18 column. The overall recovery was 83.5 +/- 2.6%, with an overall relative standard deviation of 9%.

摘要

已开发出一种高效液相色谱法，用于测定鸡肝中低至0.5 ppm水平的游离棉酚。在抗坏血酸存在下，用乙腈使组织脱蛋白，滤液用盐酸水解。释放出的纯棉酚用氯仿萃取，并在10微米的C18柱上进行梯度洗脱分析。总回收率为83.5 +/- 2.6%，总相对标准偏差为9%。