Science. 1994 Jul 15;265(5170):361-4. doi: 10.1126/science.265.5170.361.
The room temperature dynamics of single sulforhodamine 101 molecules dispersed on a glass surface are investigated on two different time scales with near-field optics. On the 10(-2)- to 10(2)-second time scale, intensity fluctuations in the emission from single molecules are examined with polarization measurements, providing insight into their spectroscopic properties. On the nanosecond time scale, the fluorescence lifetimes of single molecules are measured, and their excited-state energy transfer to the aluminum coating of the near-field probe is characterized. A movie of the time-resolved emission demonstrates the feasibility of fluorescence lifetime imaging with single molecule sensitivity, picosecond temporal resolution, and a spatial resolving power beyond the diffraction limit.
用近场光学技术研究了分散在玻璃表面的单个磺基罗丹明 101 分子在两个不同时间尺度上的室温动力学。在 10(-2)-到 10(2)-秒的时间尺度上，通过偏振测量研究了单分子发射的强度波动，从而深入了解了它们的光谱特性。在纳秒时间尺度上，测量了单分子的荧光寿命，并对其激发态能量向近场探针的铝涂层的转移进行了表征。时间分辨发射的电影证明了具有单分子灵敏度、皮秒时间分辨率和超越衍射极限的空间分辨率的荧光寿命成像的可行性。
