Suttie J M, Fennessy P F, Crosbie S F, Corson I D, Laas F J, Elgar H J, Lapwood K R
Invermay Agricultural Centre, Mosgiel, New Zealand.
J Endocrinol. 1991 Dec;131(3):467-74. doi: 10.1677/joe.0.1310467.
Blood samples were taken from six tame red deer stags at 3-15 months of age once a month from a jugular catheter every 30 min for 24 h to investigate hormonal secretion during puberty and during growth of the pedicle and first antler. All plasma samples were analysed for LH and testosterone concentrations and the resultant data were analysed using the PULSAR pulse detection routine. In addition each stag was injected wih gonadotrophin-releasing hormone (GnRH; 20 ng/kg body weight) after the above samples had been taken and the bleeding regimen was continued for a further 2 h. Body weight, antler size and status (i.e. whether the stags had a pedicle or antler) were also recorded. The pulsatile secretion of LH could be considered to have occurred in three phases. The first of these was one of development, with the LH pulse frequency increasing to 8 pulses/24 h, the second a phase of regression, with a decrease in LH pulse frequency to 2 pulses/24 h, and finally a second phase of development characterized by increased LH pulse frequency to 12 pulses/24 h. Testosterone secretion generally followed the same pattern. During the period before the permanent bony pedicles grew, there were less than five LH pulses/24 h. When the pedicles were growing, LH and testosterone pulsatile secretion increased but the pulse frequency of both hormones fell during velvet antler growth.(ABSTRACT TRUNCATED AT 250 WORDS)
从6只3至15月龄的圈养马鹿雄鹿身上采集血样，每月一次，通过颈静脉导管，每30分钟采集一次，共采集24小时，以研究青春期以及鹿茸和初角生长期间的激素分泌情况。对所有血浆样本进行促黄体生成素（LH）和睾酮浓度分析，并使用PULSAR脉冲检测程序对所得数据进行分析。此外，在采集上述样本后，给每只雄鹿注射促性腺激素释放激素（GnRH；20纳克/千克体重），并继续采血2小时。还记录了体重、鹿角大小和状态（即雄鹿是否有鹿茸或角）。LH的脉冲式分泌可分为三个阶段。第一阶段是发育阶段，LH脉冲频率增加到8次/24小时；第二阶段是衰退阶段，LH脉冲频率降至2次/24小时；最后是第二个发育阶段，其特征是LH脉冲频率增加到12次/24小时。睾酮分泌通常遵循相同模式。在永久性骨质鹿茸生长之前，每24小时的LH脉冲少于5次。当鹿茸生长时，LH和睾酮的脉冲式分泌增加，但在鹿茸生长的鹿茸期，两种激素的脉冲频率下降。（摘要截短于250字）
