Motta M C, Saraiva E M, Costa e Silva Filho F, de Souza W
Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, Brasil.
Microbios. 1991;68(275):87-96.
The surface charge of Crithidia fasciculata and Crithidia luciliae was analysed by measurement of the zeta-potential and labelling of the protozoan surface with cationized ferritin particles. Both trypanosomatids have a net negative surface charge, with a zeta-potential of -10.39 mV and -11.12 mV for C. luciliae and C. fasciculata, respectively. Enzyme treatment showed that phosphate groups, but not sialic acid, significantly contributed to the negative surface charge. Lectin-induced agglutination was used to analyse the presence of surface-exposed carbohydrates in C. fasciculata and C. luciliae. The cells did not agglutinate when incubated in the presence of lectins which recognized L-fucose, N-acetyl-D-glucosamine and sialic acid. However, lectins which bind to N-acetyl-D-galactosamine, D-galactose and D-mannose agglutinated both protozoa.
通过测量ζ电位以及用阳离子铁蛋白颗粒标记原生动物表面，对 fasciculata 克氏锥虫和 luciliae 克氏锥虫的表面电荷进行了分析。两种锥虫均具有净负表面电荷，luciliae 克氏锥虫和 fasciculata 克氏锥虫的ζ电位分别为 -10.39 mV 和 -11.12 mV。酶处理表明，磷酸基团而非唾液酸对负表面电荷有显著贡献。凝集素诱导的凝集用于分析 fasciculata 克氏锥虫和 luciliae 克氏锥虫表面暴露碳水化合物的存在情况。当在识别 L-岩藻糖、N-乙酰-D-葡萄糖胺和唾液酸的凝集素存在下孵育时，细胞不会凝集。然而，与 N-乙酰-D-半乳糖胺、D-半乳糖和 D-甘露糖结合的凝集素会使两种原生动物凝集。
