Parmley R T, Spicer S S, Garvin A J
Cancer Res. 1976 May;36(5):1717-24.
A multilaminar alteration of endoplasmic reticulum (ER) has been observed in tumor cells of eight patients with Hodgkin's disease and a patient with histiocytic lymphoma. These multilaminar structures are more numerous in dividing cells and thus appear to arise primarily during mitosis. The stacked membranes in the multilaminar structures possibly result from abnormal sticking of organelle membranes, as evidenced in this study of adherence of ER to other elements of ER, nuclear envelope, mitochondria, or lipid droplets. Multilaminar ER was identified in all mitotic tumor cells, a rare mitotic plasma cell, and numerous interphase Hodgkin cells. The paucity of multilaminar ER in normal mitotic cells and its virtual absence for normal interphase cells suggest that this structure represents a pathological alteration in tumor cells from patients with Hodgkin's disease and histiocytic lymphoma. The multilaminar defect of ER is associated with other atypical features of ER in Hodgkin tumor cells, including the excessive length and curving of ER profiles, the collapse of the ER cisternae, and the overall sparsity of this organelle. Other abnormalities observed in mitotic Hodgkin tumor cells include the presence of disorganized microtubules, large cytoplasmic vacuoles, and abnormally clumped chromosomal material and the persistence throughout mitosis of bodies suggestive of nucleoli and of the nuclear bodies of interphase cells.
在8例霍奇金病患者和1例组织细胞淋巴瘤患者的肿瘤细胞中观察到内质网（ER）的多层改变。这些多层结构在分裂细胞中更为常见，因此似乎主要在有丝分裂期间出现。多层结构中的堆叠膜可能是由于细胞器膜的异常粘连所致，本研究中内质网与内质网的其他成分、核膜、线粒体或脂滴的粘连情况证明了这一点。在所有有丝分裂肿瘤细胞、罕见的有丝分裂浆细胞以及众多间期霍奇金细胞中均发现了多层内质网。正常有丝分裂细胞中多层内质网较少，正常间期细胞中几乎不存在，这表明这种结构代表了霍奇金病和组织细胞淋巴瘤患者肿瘤细胞的一种病理改变。内质网的多层缺陷与霍奇金肿瘤细胞内质网的其他非典型特征有关，包括内质网轮廓过长和弯曲、内质网池塌陷以及该细胞器整体稀疏。在有丝分裂的霍奇金肿瘤细胞中观察到的其他异常包括微管排列紊乱、大的细胞质空泡、染色体物质异常聚集，以及在整个有丝分裂过程中存在提示核仁和间期细胞核体的结构。
