Kumar Sunil, Chaudhury Koel, Sen Prasenjit, Guha Sujoy K
School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal 721 302, India.
J Microsc. 2007 Aug;227(Pt 2):118-23. doi: 10.1111/j.1365-2818.2007.01795.x.
A new male contraceptive given the name RISUG (an acronym for Reversible Inhibition of Sperm Under Guidance) has been developed by our research group. RISUG is a bioactive polymer and is injected into the lumen of the vas deferens using a no-scalpel approach. The polyelectrolytic nature of this contraceptive induces a surface charge imbalance on sperm membrane system leading to its destabilization. Complete disintegration of the plasma membrane with subsequent rupture and dispersion of the acrosomal contents is observed on RISUG treatment. In the present study, micro-structural properties of human spermatozoa exposed to RISUG in vitro have been quantitatively analysed using atomic force microscopy. The parameters used to quantify these morphological changes include amplitude (peak-valley height difference, arithmetic roughness, root mean square roughness) and spatial roughness. Factor loadings (Varimax rotation) have been used to determine the parameters displaying maximum variation. Further, sperm cells have been classified in various principal-component planes using principal-component analysis. The periodic structural features of the atomic force microscopy images have also been obtained using power spectral analysis.
我们的研究小组研发出了一种名为RISUG（引导下精子可逆抑制的首字母缩写）的新型男性避孕药。RISUG是一种生物活性聚合物，采用无手术刀方法注入输精管腔。这种避孕药的聚电解质性质会导致精子膜系统表面电荷失衡，从而使其不稳定。在RISUG处理后，可以观察到质膜完全解体，随后顶体内容物破裂并分散。在本研究中，使用原子力显微镜对体外暴露于RISUG的人类精子的微观结构特性进行了定量分析。用于量化这些形态变化的参数包括振幅（峰谷高差、算术粗糙度、均方根粗糙度）和空间粗糙度。因子载荷（方差最大化旋转）已用于确定显示最大变化的参数。此外，使用主成分分析将精子细胞分类到各个主成分平面中。还使用功率谱分析获得了原子力显微镜图像的周期性结构特征。
