HIV-1在静止细胞中的中心体整合前潜伏期。
Centrosomal pre-integration latency of HIV-1 in quiescent cells.
作者信息
Zamborlini Alessia, Lehmann-Che Jacqueline, Clave Emmanuel, Giron Marie-Lou, Tobaly-Tapiero Joëlle, Roingeard Philippe, Emiliani Stéphane, Toubert Antoine, de Thé Hugues, Saïb Ali
机构信息
CNRS UMR7151, Université Paris 7, Hôpital Saint-Louis, Paris, France.
出版信息
Retrovirology. 2007 Sep 10;4:63. doi: 10.1186/1742-4690-4-63.
Abstract
Human immunodeficiency virus type 1 (HIV-1) efficiently replicates in dividing and non-dividing cells. However, HIV-1 infection is blocked at an early post-entry step in quiescent CD4+ T cells in vitro. The molecular basis of this restriction is still poorly understood. Here, we show that in quiescent cells, incoming HIV-1 sub-viral complexes concentrate and stably reside at the centrosome for several weeks. Upon cell activation, viral replication resumes leading to viral gene expression. Thus, HIV-1 can persist in quiescent cells as a stable, centrosome-associated, pre-integration intermediate.
摘要
1型人类免疫缺陷病毒（HIV-1）能在分裂细胞和非分裂细胞中高效复制。然而，在体外，HIV-1感染在静止CD4+ T细胞进入后的早期步骤就被阻断。这种限制的分子基础仍知之甚少。在此，我们表明，在静止细胞中，进入的HIV-1亚病毒复合物聚集并稳定地驻留在中心体数周。细胞激活后，病毒复制恢复，导致病毒基因表达。因此，HIV-1可以作为一种稳定的、与中心体相关的整合前中间体在静止细胞中持续存在。
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