Shen Dan-hua, Wang Feng-hua, Yu You-zhi
Department of Pathology, People's Hospital, Peking University, Beijing 100044, China.
Zhonghua Bing Li Xue Za Zhi. 2007 Jul;36(7):457-60.
To evaluate the sensitivity and specificity of chromogenic in-situ hybridization (CISH) in detecting HER2 gene amplification in breast carcinomas.
HER2 oncogene amplification and its protein expression in 165 cases of breast carcinoma were investigated by immunohistochemistry (IHC) and CISH.
(1) CISH did not detect HER2 gene amplification in 107 cases of IHC negative tumors and 24 cases of IHC 1+ tumors. (2) CISH identified high copy numbers of HER2 gene amplification in 21/22 (95.5%) cases with IHC 3+. (3) In 12 HIC 2+ cases, CISH identified 3 cases of high copy number amplification, 6 cases of low copy number amplification and 3 cases without amplification.
HER2 gene amplification detection by CISH is highly sensitive and has a high concordance with IHC detection of the protein expression. It is concluded that CISH is a tool to evaluate HER2 gene status in breast cancer and can be an implement in conventional pathology laboratories.
评估显色原位杂交（CISH）检测乳腺癌中HER2基因扩增的敏感性和特异性。
采用免疫组织化学（IHC）和CISH检测165例乳腺癌中HER2癌基因扩增及其蛋白表达情况。
（1）CISH在107例IHC阴性肿瘤和24例IHC 1+肿瘤中未检测到HER2基因扩增。（2）CISH在21/22（95.5%）例IHC 3+病例中检测到HER2基因高拷贝数扩增。（3）在12例IHC 2+病例中，CISH检测到3例高拷贝数扩增、6例低拷贝数扩增和3例无扩增。
CISH检测HER2基因扩增具有高度敏感性，且与IHC检测蛋白表达具有高度一致性。得出结论，CISH是评估乳腺癌中HER2基因状态的一种工具，可作为常规病理实验室的一种检测手段。
