利用反相高效液相色谱法和质谱法对山羊β-酪蛋白的研究：一种新遗传变异体的鉴定

Study of caprine beta-casein using reversed-phase high-performance liquid chromatography and mass spectroscopy: identification of a new genetic variant.

作者信息

Moatsou Golfo, Mollé Daniel, Moschopoulou Ekaterini, Valérie Gagnaire

机构信息

Laboratory of Dairy Science and Technology, Department of Food Science and Technology, Agricultural University of Athens, Iera Odos 75, Athens, 118 55, Greece.

出版信息

Protein J. 2007 Dec;26(8):562-8. doi: 10.1007/s10930-007-9098-8.

DOI:10.1007/s10930-007-9098-8

PMID:17846874

Abstract

The genotypes and the main phosphorylation levels of beta-casein of goat milk were studied using RP-HPLC/ESI-MS. A new variant of caprine beta-casein named E has been characterized using RP-HPLC/ESI-MS, MALDI-MS and NanoESI MS/MS methods. Its sequence differed from that of variant A in the mono amino acid substitution D47 --> Y47, which resulted in a 48 Da experimental mass difference between them. The calculated molecular mass of the new variant E 6 P was estimated as 23,869 Da. Its phosphorylation pattern was similar to that of variant A, the most abundant types being those with 5 and 6 P in similar quantities.

摘要

采用反相高效液相色谱/电喷雾电离质谱法（RP-HPLC/ESI-MS）研究了山羊奶β-酪蛋白的基因型和主要磷酸化水平。利用RP-HPLC/ESI-MS、基质辅助激光解吸电离质谱法（MALDI-MS）和纳升电喷雾串联质谱法（NanoESI MS/MS）对一种名为E的山羊β-酪蛋白新变体进行了表征。其序列与A变体的不同之处在于单个氨基酸取代D47→Y47，这导致它们之间的实验质量差为48 Da。新变体E 6P的计算分子量估计为23869 Da。其磷酸化模式与A变体相似，最丰富的类型是含5个和6个磷酸基团且数量相似的类型。