人卵丘细胞中连接蛋白43的减少在卵胞浆内单精子注射期间产生良好的胚胎发育潜能。
Reduction of connexin 43 in human cumulus cells yields good embryo competence during ICSI.
作者信息
Hasegawa Junichi, Yanaihara Atsushi, Iwasaki Shinji, Mitsukawa Kaori, Negishi Momoko, Okai Takashi
机构信息
Department of Obstetrics and Gynecology, Showa University School of Medicine, 1-5-8, Hatanodai, Shinagawa-ku, Tokyo 142-8666, Japan.
出版信息
J Assist Reprod Genet. 2007 Oct;24(10):463-6. doi: 10.1007/s10815-007-9155-4. Epub 2007 Sep 1.
Abstract
PURPOSE
The purpose of this study was to predict developmental competence of human oocytes during ICSI via analysis of connexin 43 (Cx43) in cumulus cells surrounding mature oocytes.
MATERIALS AND METHODS
Human cumulus cells were manually separated from the oocyte-cumulus complex under a microscope. Cx43 mRNA was expressed by real-time quantitative polymerase chain reaction (RT-PCR) measurement in cumulus cells.
RESULTS
There was no significant relationship between expression of Cx43 and fertilisation or cleavage rate. However, Cx43 expression was lower in the good morphology group (blastomeres>7 cells with fragmentation<10% on day 3) when compared to the other groups (p=0.035).
CONCLUSIONS
These results suggest that full reduction of Cx43 expression on cumulus cells at the time of oocyte collection during ICSI is essential for developmental competence of human oocytes.
摘要
目的
本研究的目的是通过分析成熟卵母细胞周围卵丘细胞中的连接蛋白43（Cx43）来预测ICSI过程中人类卵母细胞的发育能力。
材料与方法
在显微镜下手动将人类卵丘细胞与卵母细胞-卵丘复合体分离。通过实时定量聚合酶链反应（RT-PCR）测量卵丘细胞中Cx43 mRNA的表达。
结果
Cx43的表达与受精或分裂率之间无显著关系。然而，与其他组相比，形态良好组（第3天卵裂球>7个且碎片<10%）中Cx43的表达较低（p = 0.035）。
结论
这些结果表明，在ICSI过程中收集卵母细胞时，卵丘细胞上Cx43表达的完全降低对人类卵母细胞的发育能力至关重要。
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