Okazaki Tetsuji, Nishibori Masahide, Yamashita Yasuhisa, Shimada Masayuki
Laboratory of Animal Reproduction, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8528, Japan.
Mol Cell Endocrinol. 2003 Nov 14;209(1-2):43-50. doi: 10.1016/j.mce.2003.08.002.
It has been reported that LH receptor (LHR) mRNA is not detected in cumulus cells of porcine cumulus-oocyte complexes (COCs) just after collection from small antral follicles. The present study showed that the formation of LHR in cumulus cells was up-regulated by the cultivation with 20 ng/ml FSH. When the newly synthesized receptors were stimulated by 1.0 microg/ml LH, significantly higher levels of cAMP and progesterone production in cumulus cells were observed as compared with those of COCs cultured with FSH. A loss of proliferative activity of cumulus cells was induced by the additional LH to FSH-containing medium; however, the inhibitory effect was overcome by progesterone receptor antagonist RU486. Furthermore, the addition of LH also accelerated ongoing GVBD in cumulus cells-enclosed oocytes. These results revealed that during in vitro meiotic maturation of porcine COCs, progesterone secreted by FSH- and LH-stimulated cumulus cells reduced proliferative activity of cumulus cells; the changes of cumulus cells might be involved in inducing meiotic resumption of porcine oocytes.
据报道，从小卵泡采集的猪卵丘-卵母细胞复合体（COCs）的卵丘细胞中，刚采集后检测不到促黄体生成素受体（LHR）mRNA。本研究表明，用20 ng/ml促卵泡素（FSH）培养可上调卵丘细胞中LHR的形成。当用1.0 μg/ml促黄体生成素（LH）刺激新合成的受体时，与用FSH培养的COCs相比，卵丘细胞中观察到显著更高水平的环磷酸腺苷（cAMP）和孕酮产生。向含FSH的培养基中额外添加LH会诱导卵丘细胞增殖活性丧失；然而，孕酮受体拮抗剂RU486可克服这种抑制作用。此外，添加LH还加速了卵丘细胞包裹的卵母细胞中正在进行的生发泡破裂（GVBD）。这些结果表明，在猪COCs的体外减数分裂成熟过程中，FSH和LH刺激的卵丘细胞分泌的孕酮降低了卵丘细胞的增殖活性；卵丘细胞的这些变化可能参与诱导猪卵母细胞减数分裂恢复。
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