Kelly Tamsin, Doble Philip, Dawson Michael
Centre for Forensic Science, Faculty of Science, University of Technology, Sydney, NSW, Australia.
Electrophoresis. 2007 Oct;28(19):3566-9. doi: 10.1002/elps.200700090.
The utilization of dynamic doubly coated capillaries for a fast separation of methadone and its two major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline (EMDP) was investigated. The coated capillaries were prepared using a polycation of poly(diallyldimethylammonium chloride) and a polyanion of dextran sulfate. A fast achiral separation was developed using the coated capillaries with a BGE of 100 mM phosphate buffer at pH 2.6. Complete achiral separation of methadone, EDDP and EMDP was achieved, with migration times of approximately 4 min. The method offers considerable advantages with respect to BGE simplicity and analysis time compared to previously published CE methods for methadone and its related analytes.
研究了使用动态双涂层毛细管快速分离美沙酮及其两种主要代谢物，即2-亚乙基-1,5-二甲基-3,3-二苯基吡咯烷（EDDP）和2-乙基-5-甲基-3,3-二苯基-1-吡咯啉（EMDP）。使用聚二烯丙基二甲基氯化铵的聚阳离子和硫酸葡聚糖的聚阴离子制备涂层毛细管。使用涂层毛细管和pH为2.6的100 mM磷酸盐缓冲液作为背景电解质（BGE）开发了一种快速非手性分离方法。实现了美沙酮、EDDP和EMDP的完全非手性分离，迁移时间约为4分钟。与先前发表的用于美沙酮及其相关分析物的毛细管电泳（CE）方法相比，该方法在BGE简单性和分析时间方面具有相当大的优势。
