Woo Jeong-Soo, Kim Kyoung-Min, Kang Jae Seong, Zodpe Prakash, Chae Sung-Won, Hwang Soon-Jae, Lee Heung-Man
From the Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Korea.
Ann Otol Rhinol Laryngol. 2007 Aug;116(8):599-603. doi: 10.1177/000348940711600808.
We performed an observational study of RNA and protein expression in human tissue to examine the distribution of neutrophil gelatinase-associated lipocalin (NGAL) in normal and chronic inflammatory salivary tissues, and to investigate the expression level of NGAL in inflammatory conditions of salivary glands.
Normal salivary gland tissues and tissue samples of salivary glands with chronic sialadenitis were obtained. Expression of NGAL was investigated by reverse transcriptase-polymerase chain reaction, and semiquantitative analysis of these results was also performed. The differential localization and amount of immunoreactivity to NGAL protein was evaluated by immunohistochemistry and Western blot analysis in normal salivary gland tissues and salivary glands with chronic sialadenitis.
NGAL messenger RNA transcripts were detected in the tissues from the salivary glands with chronic sialadenitis, but only a small amount was detected in the tissues from the normal salivary glands. A weak expression of NGAL protein was occasionally seen in a few ductal epithelial cells of normal salivary gland tissue. However, in tissue samples from glands with chronic sialadenitis, the NGAL protein was expressed strongly in ductal epithelial cells and infiltrating inflammatory cells.
These results imply that NGAL is associated with the regulation of inflammation in salivary glands.
我们对人体组织中的RNA和蛋白质表达进行了一项观察性研究，以检查中性粒细胞明胶酶相关脂质运载蛋白（NGAL）在正常和慢性炎症性唾液腺组织中的分布，并研究唾液腺炎症状态下NGAL的表达水平。
获取正常唾液腺组织和慢性涎腺炎唾液腺组织样本。通过逆转录聚合酶链反应研究NGAL的表达，并对这些结果进行半定量分析。通过免疫组织化学和蛋白质印迹分析评估正常唾液腺组织和慢性涎腺炎唾液腺中NGAL蛋白的差异定位和免疫反应性数量。
在慢性涎腺炎唾液腺组织中检测到NGAL信使核糖核酸转录本，但在正常唾液腺组织中仅检测到少量。在正常唾液腺组织的少数导管上皮细胞中偶尔可见NGAL蛋白的弱表达。然而，在慢性涎腺炎腺体的组织样本中，NGAL蛋白在导管上皮细胞和浸润性炎症细胞中强烈表达。
这些结果表明NGAL与唾液腺炎症的调节有关。
