Wani Parvaze Ahmad, Khan Md Saghir, Zaidi Almas
Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.
Biotechnol Lett. 2008 Jan;30(1):159-63. doi: 10.1007/s10529-007-9515-2. Epub 2007 Sep 12.
Mesorhizobium strain RC3, isolated from chickpea nodules, tolerated chromium up to 500 mug/ml and reduced it by 90% at pH 7 after 120 h. It produced plant growth-promoting substances, both in the presence and absence of chromium. Strain RC3 produced 35 mug indole acetic acid/ml in Luria Bertani broth with 100 mg tryptophan/ml, which decreased with an increase in chromium concentration. Chromium application to soil at 136 mg/kg was toxic to chickpea plants but when RC3 at 136 mg/kg was also added, it increased the dry matter accumulation, number of nodules, seed yield and grain protein by 71, 86, 36 and 16%, respectively, compared to non-inoculated plants. Nitrogen in roots and shoots were increased by 46 and 40%, respectively, at 136 mg Cr/kg. The bio-inoculant decreased the uptake of chromium by 14, 34 and 29% in roots, shoots and grains, respectively.
从鹰嘴豆根瘤中分离出的中生根瘤菌菌株RC3，能耐受高达500微克/毫升的铬，并在pH值为7、120小时后将其还原90%。无论有无铬存在，该菌株都能产生促进植物生长的物质。菌株RC3在含有100毫克/毫升色氨酸的Luria Bertani肉汤中产生35微克/毫升的吲哚乙酸，其含量随铬浓度的增加而降低。在土壤中施加136毫克/千克的铬对鹰嘴豆植株有毒，但当同时添加136毫克/千克的RC3时，与未接种的植株相比，干物质积累、根瘤数量、种子产量和籽粒蛋白质分别增加了71%、86%、36%和16%。在铬含量为136毫克/千克时，根和地上部的氮含量分别增加了46%和40%。这种生物接种剂分别使根、地上部和籽粒对铬的吸收减少了14%、34%和29%。
