Endo Masayuki, Fujitsuka Mamoru, Majima Tetsuro
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
Chemistry. 2007;13(31):8660-6. doi: 10.1002/chem.200700895.
We have demonstrated the construction of multiple porphyrin arrays in the tobacco mosaic virus (TMV) supramolecular structures by self-assembly of recombinant TMV coat protein (TMVCP) monomers, in which Zn-coordinated porphyrin (ZnP) and free-base porphyrin (FbP) were site-selectively incorporated. The photophysical properties of porphyrin moieties incorporated in the TMV assemblies were also characterized. TMV-porphyrin conjugates employed as building blocks self-assembled into unique disk and rod structures under the proper conditions as similar to native TMV assemblies. The mixture of a ZnP donor and an FbP acceptor was packed in the TMV assembly and showed energy transfer and light-harvesting activity. The detailed photophysical properties of the arrayed porphyrins in the TMV assemblies were examined by time-resolved fluorescence spectroscopy, and the energy transfer rates were determined to be 3.1-6.4x10(9) s(-1). The results indicate that the porphyrins are placed at the expected positions in the TMV assemblies.
我们通过重组烟草花叶病毒（TMV）衣壳蛋白（TMVCP）单体的自组装，展示了在烟草花叶病毒超分子结构中构建多个卟啉阵列，其中锌配位卟啉（ZnP）和游离碱卟啉（FbP）被位点选择性地掺入。还对掺入TMV组装体中的卟啉部分的光物理性质进行了表征。作为构建模块的TMV-卟啉共轭物在适当条件下自组装成独特的盘状和棒状结构，类似于天然TMV组装体。ZnP供体和FbP受体的混合物被包装在TMV组装体中，并表现出能量转移和光捕获活性。通过时间分辨荧光光谱研究了TMV组装体中排列的卟啉的详细光物理性质，确定能量转移速率为3.1-6.4×10⁹ s⁻¹。结果表明，卟啉被放置在TMV组装体中的预期位置。
