Amoudy H A, Ahmad S, Thole J E, Mustafa A S
Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Scand J Immunol. 2007 Oct;66(4):422-5. doi: 10.1111/j.1365-3083.2007.01961.x.
Previously we identified a novel antigenic open reading frame (ORF), designated as ORF-14, on the RD1 region of Mycobacterium tuberculosis that was not originally predicted by Mahairas or by annotation of the M. tuberculosis H37 Rv genome. Here we show that anti-ORF-14 antibodies either from mice immunized with recombinant ORF-14 protein or isolated from serum samples from tuberculosis patients, react with a protein in culture filtrate but not in cytoplasmic or cell wall fractions from M. tuberculosis. Our data indicate that the ORF-14 protein is expressed as a secreted protein, representing one more secreted protein antigen not previously identified by genomics.
此前，我们在结核分枝杆菌的RD1区域鉴定出一个新的抗原性开放阅读框（ORF），命名为ORF-14，该区域最初并未被Mahairas预测，也未在结核分枝杆菌H37 Rv基因组注释中出现。在此，我们发现，用重组ORF-14蛋白免疫的小鼠产生的抗ORF-14抗体，或从肺结核患者血清样本中分离出的抗ORF-14抗体，能与培养滤液中的一种蛋白发生反应，但不能与结核分枝杆菌的细胞质或细胞壁组分中的蛋白发生反应。我们的数据表明，ORF-14蛋白作为一种分泌蛋白表达，这代表了一种此前未通过基因组学鉴定出的分泌蛋白抗原。
