Wang Na, Wang Gui-ying, Guo Wei, Dong Xiu-juan, Li Yan
Division of Molecular Biology, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 Apr;28(4):394-7.
To study the relation between single nucleotide polymorphism(SNP) at the 91T-->A(Phe31Ile) position of the STK15 gene and the susceptibility of esophageal squamous cell carcinoma (ESCC) in She county--a ESCC high incidence region in North China.
Polymerase-chain reaction(PCR)-restriction fragment length polymorphism (RFLP) analysis was used to detect the genotypes of STKl5 Phe31Ile(91T-->A) SNP, and the samples came from 296 ESCC patients and 302 healthy controls.
The risk of ESCC significantly increased in the group which had been smoking or having a family history of upper gastrointestinal cancer (UGIC) (the OR = 1.68 and 1.77, 95% CI: 1.34-2.10 and 1.44-2.19, respectively). Rates of the three genotypes (Phe/Phe, Phe/Ile, Ile/Ile) of the STK15 Phe31Ile (91T-->A) SNPs in ESCC patients were 11.5%, 34.8% and 53.7%, respectively, and were not significantly different from that in the healthy group (11.9%, 36.8% and 51.3%) (chi2 = 0.35, P = 0.84). When compared to Phe/Phe genotype, Phe/Ile and Ile/Ile of STK15 91T-->A(Phe31Ile)did not show effect on the risk of ESCC according to the odds ratio results which were 0.98 (95% CI: 0.57-1.69) and 1.09 (0.65-1.82) respectively. STK15 91T-->A (Phe31Ile) SNP also did not significantly influence on the development of ESCC even the samples were stratified by sex, smoking status and family history of upper gastrointestinal cancer.
The STK15 Phe31Ile(91T-->A) polymorphisms seemed irrelevant with the risk of ESCC in She county.
研究STK15基因91T→A（Phe31Ile）位点单核苷酸多态性（SNP）与中国北方食管癌高发区涉县食管鳞状细胞癌（ESCC）易感性的关系。
采用聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）分析检测STKl5 Phe31Ile（91T→A）SNP的基因型，样本来自296例ESCC患者和302例健康对照。
有吸烟史或有上消化道癌（UGIC）家族史的人群患ESCC的风险显著增加（OR分别为1.68和1.77，95%CI：1.34 - 2.10和1.44 - 2.19）。ESCC患者中STK15 Phe31Ile（91T→A）SNP的三种基因型（Phe/Phe、Phe/Ile、Ile/Ile）频率分别为11.5%、34.8%和53.7%，与健康组（11.9%、36.8%和51.3%）相比无显著差异（χ2 = 0.35，P = 0.84）。根据比值比结果，与Phe/Phe基因型相比，STK15 91T→A（Phe31Ile）的Phe/Ile和Ile/Ile基因型对ESCC风险无影响，比值比分别为0.98（95%CI：0.57 - 1.69）和1.09（0.65 - 1.82）。即使按性别、吸烟状况和上消化道癌家族史对样本进行分层，STK15 91T→A（Phe31Ile）SNP对ESCC的发生也无显著影响。
STK15 Phe31Ile（91T→A）多态性似乎与涉县ESCC的风险无关。
