Liu Fu-Ken
Department of Applied Chemistry, National University of Kaohsiung, No. 700, Kaohsiung University Rd., Nan Tzu District, Kaohsiung 811, Taiwan.
J Chromatogr A. 2007 Oct 12;1167(2):231-5. doi: 10.1016/j.chroma.2007.08.058. Epub 2007 Aug 31.
In this paper, we present a highly efficient capillary electrophoresis (CE)-based method for characterizing the sizes of Au nanoparticles (NPs). We used the reversed electrode polarity stacking mode (REPSM) of CE for on-line enhancement of the detection and separation of Au NPs. Under the on-line enhancement separation conditions [buffer: SDS (70 mM) and 3-cyclohexylamino-1-propanesulfonic acid (10 mM) at pH 10.0; applied voltage: 20 kV; using the REPSM for sample concentration], the detection sensitivity toward the Au NPs improved by up to 260-fold. A linear relationship (R(2)=0.994) existed between the electrophoretic mobilities and the sizes of the Au NPs within the range of diameters from 5.3 to 59.9 nm; the relative standard deviations of the electrophoretic mobilities for these NPs were below 0.6%. When using these conditions to analyze Au NPs produced through seed-assisted synthesis, a good correlation existed between the sizes obtained using CE and those provided by transmission electron microscopy. Furthermore, the analyses performed using CE were rapid (<4 min) and required very low sample volumes (several nanoliters), yet they proceeded with high sensitivity. These findings confirm that CE is an efficient tool for characterizing the sizes of NPs.
在本文中，我们提出了一种基于高效毛细管电泳（CE）的方法来表征金纳米颗粒（NPs）的尺寸。我们使用CE的反向电极极性堆积模式（REPSM）对金纳米颗粒进行在线增强检测和分离。在在线增强分离条件下[缓冲液：pH值为10.0的70 mM十二烷基硫酸钠（SDS）和10 mM 3-环己基氨基-1-丙烷磺酸（CAPS）；施加电压：20 kV；使用REPSM进行样品浓缩]，对金纳米颗粒的检测灵敏度提高了260倍。在直径为5.3至59.9 nm的范围内，金纳米颗粒的电泳迁移率与其尺寸之间存在线性关系（R(2)=0.994）；这些纳米颗粒电泳迁移率的相对标准偏差低于0.6%。当使用这些条件分析通过种子辅助合成产生的金纳米颗粒时，CE获得的尺寸与透射电子显微镜提供的尺寸之间存在良好的相关性。此外，使用CE进行分析速度很快（<4分钟），所需样品体积非常小（几纳升），但灵敏度很高。这些结果证实CE是表征纳米颗粒尺寸有效的工具。
