Decaro Nicola, Desario Costantina, Lucente Maria Stella, Amorisco Francesca, Campolo Marco, Elia Gabriella, Cavalli Alessandra, Martella Vito, Buonavoglia Canio
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy.
J Virol Methods. 2008 Jan;147(1):67-71. doi: 10.1016/j.jviromet.2007.08.006. Epub 2007 Sep 11.
Taking into account reports of the isolation of canine parvoviruses (CPVs) from faecal samples of cats, we developed a real-time PCR assay, based on minor groove binder (MGB) probe technology, for rapid discrimination between true feline panleukopenia viruses (FPLVs) from CPVs. The assay takes advantage of a single nucleotide polymorphism at position 3753 of the viral genome (corresponding to residue 323 of the capsid VP2 protein) and of the ability of MGB probes to bind specifically only to perfectly complementary sequences. The FPV/CPV assay was proven to be highly specific, sensitive and reproducible and correlated well with a TaqMan assay able to recognise canine as well as feline parvoviruses. Using this assay for extensive molecular surveys will provide precise information on the real circulation of the CPV antigenic variants, including the new variant 2c, in cat population worldwide.
考虑到从猫的粪便样本中分离出犬细小病毒（CPV）的报告，我们基于小沟结合物（MGB）探针技术开发了一种实时PCR检测方法，用于快速区分真正的猫泛白细胞减少症病毒（FPLV）和CPV。该检测方法利用了病毒基因组第3753位的单核苷酸多态性（对应衣壳VP2蛋白的第323位残基）以及MGB探针仅能特异性结合完全互补序列的能力。经证实，FPV/CPV检测方法具有高度特异性、敏感性和可重复性，并且与能够识别犬和猫细小病毒的TaqMan检测方法相关性良好。使用该检测方法进行广泛的分子调查将提供有关CPV抗原变异体（包括新的2c变异体）在全球猫群体中实际传播情况的精确信息。
