Bae Eun Hui, Kim In Jin, Park Jeong Woo, Ma Seong Kwon, Choi Ki Chul, Lee Jong Un, Kim Soo Wan
Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea.
Urol Int. 2007;79(2):170-6. doi: 10.1159/000106333.
The present study was designed to investigate the role of the local renin-angiotensin-aldosterone system (RAAS), endothelin (ET) and the natriuretic peptide system (NPS) for the development of renal fibrosis and progressive renal disease in experimental unilateral ureteral obstructed (UUO) rats.
Male Sprague-Dawley rats (180-200 g) were unilaterally obstructed by ligation of the proximal ureters for 14 days. Control rats were treated in the same way, except that no ligature was made. The mRNA expressions of local renin-angiotensin system, aldosterone synthase (CYP11B2), ET-1 and NPS was determined in the cortex by real-time polymerase chain reaction.
Following the unilateral ureteral obstruction, the mRNA expressions of angiotensin-converting enzyme 1, ET-1 and transforming growth factor-beta1 (TGF-beta1) were increased, while angiotensin-converting enzyme 2 was decreased in the obstructed kidney compared with the controls. Angiotensin II type 1 receptor was decreased and TGF-beta1 was not changed in contralateral kidney compared with the controls. Atrial natriuretic peptide, brain natriuretic peptide, and C-type natriuretic peptide expressions were increased in UUO kidneys compared with the controls, while natriuretic peptide receptor-A was decreased in UUO kidneys.
The local RAAS and ET-1 was upregulated which may play a role in the progressive renal fibrosis in obstructed kidneys in rats with UUO. The enhanced activity of NPS in UUO kidney may play a role to compensate against progressive renal fibrosis in chronic obstructive uropathy.
本研究旨在探讨局部肾素 - 血管紧张素 - 醛固酮系统（RAAS）、内皮素（ET）和利钠肽系统（NPS）在实验性单侧输尿管梗阻（UUO）大鼠肾纤维化和进行性肾病发展中的作用。
雄性Sprague-Dawley大鼠（180 - 200 g）通过结扎近端输尿管进行单侧梗阻14天。对照大鼠以相同方式处理，但不进行结扎。通过实时聚合酶链反应测定皮质中局部肾素 - 血管紧张素系统、醛固酮合酶（CYP11B2）、ET - 1和NPS的mRNA表达。
单侧输尿管梗阻后，与对照组相比，梗阻肾脏中血管紧张素转换酶1、ET - 1和转化生长因子 - β1（TGF - β1）的mRNA表达增加，而血管紧张素转换酶2减少。与对照组相比，对侧肾脏中血管紧张素II 1型受体减少，TGF - β1无变化。与对照组相比，UUO肾脏中的心钠素、脑钠素和C型钠尿肽表达增加，而UUO肾脏中的利钠肽受体 - A减少。
局部RAAS和ET - 1上调，这可能在UUO大鼠梗阻肾脏的进行性肾纤维化中起作用。UUO肾脏中NPS活性增强可能在慢性梗阻性尿路病中对代偿进行性肾纤维化起作用。
