Shintani Masaki, Fukushima Naoya, Tezuka Meguru, Yamane Hisakazu, Nojiri Hideaki
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
Biotechnol Lett. 2008 Jan;30(1):117-22. doi: 10.1007/s10529-007-9519-y. Epub 2007 Sep 12.
The transfer of the IncP-7 carbazole degradative plasmid pCAR1 from Pseudomonas putida SM1443 (derived from strain KT2440) into bacteria of river water samples was monitored using a reporter gene encoding red fluorescent protein (RFP). The number of transconjugants drastically increased in the presence of carbazole, and most appeared to belong to the genus Pseudomonas. The results suggest that the presence of carbazole benefits the appearance of transconjugants belonging to the genus Pseudomonas. Intriguingly, we also detected the transfer of pCAR1 into non-Pseudomonas, Stenotrophomonas-like bacteria.
使用编码红色荧光蛋白（RFP）的报告基因监测了IncP - 7咔唑降解性质粒pCAR1从恶臭假单胞菌SM1443（源自菌株KT2440）向河水样本细菌中的转移。在咔唑存在的情况下，接合子的数量急剧增加，并且大多数似乎属于假单胞菌属。结果表明，咔唑的存在有利于假单胞菌属接合子的出现。有趣的是，我们还检测到pCAR1转移到了非假单胞菌属的嗜麦芽窄食单胞菌样细菌中。
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