Shintani Masaki, Yamane Hisakazu, Nojiri Hideaki
Biotechnology Research Center, The University of Tokyo, Japan.
Biosci Biotechnol Biochem. 2010;74(2):343-9. doi: 10.1271/bbb.90675. Epub 2010 Feb 7.
pCAR1 endows carbazole-degrading ability on its host. Three different host Pseudomonas (P. fluorescens Pf0-1, P. resinovorans CA10dm4, and P. chlororaphis IAM1511) were prepared to compare their behaviors in artificial microcosms (sterile soil and water supplemented with carbazole) with previous results for P. putida SM1443 (Shintani et al., Appl. Microbiol. Biotechnol., 80, 485-497 (2008)). We monitored carbazole degradation, bacterial survival, and conjugative transfer of pCAR1. Carbazole degradations were not detected with any of three hosts in the soil microcosms, but were in most of the water microcosms. Degradative efficiency varied host by host. SM1443 is an appropriate host for carbazole degradation in soil microcosms, while CA10dm4 is for water microcosms. pCAR1 transfers were detected only in the water microcosms inoculated with SM1443 or IAM1511 as the host. This is the first report to compare the behaviors of various hosts of the same plasmid in these microcosms.
pCAR1赋予其宿主咔唑降解能力。制备了三种不同的宿主假单胞菌（荧光假单胞菌Pf0-1、树脂降解假单胞菌CA10dm4和绿针假单胞菌IAM1511），并将它们在人工微宇宙（添加了咔唑的无菌土壤和水）中的行为与恶臭假单胞菌SM1443的先前结果进行比较（Shintani等人，《应用微生物学与生物技术》，80，485-497（2008年））。我们监测了咔唑降解、细菌存活以及pCAR1的接合转移情况。在土壤微宇宙中，三种宿主均未检测到咔唑降解，但在大多数水微宇宙中检测到了。降解效率因宿主而异。SM1443是土壤微宇宙中咔唑降解的合适宿主，而CA10dm4是水微宇宙中的合适宿主。仅在以SM1443或IAM1511作为宿主接种的水微宇宙中检测到了pCAR1的转移。这是首次在这些微宇宙中比较同一质粒不同宿主行为的报告。
