Department of Hematology, Zekai Tahir Burak Women's Health and Research Hospital, Ankara, Turkey.
Acta Obstet Gynecol Scand. 2007 Oct;86(10):1193-9. doi: 10.1080/00016340701552434.
Local autocrine-paracrine renin-angiotensin system (RAS), independently functioning from the circulating RAS, is present in major organs of the female reproductive tract. We have previously demonstrated 'a local RAS in human umbilical cord' via verifying the corresponding ACE, renin, and angiotensinogen mRNAs. The aim of this study is to search alterations of the local umbilical cord RAS during pre-eclampsia.
Cord blood samples were obtained from 19 patients with pre-eclampsia (aged mean 26.6 ± 5.83 (range 18-42) years) and 20 women with normal pregnancy (aged mean 28.26 ± 7.30 (range 19-37) years). Women with uncomplicated pregnancy formed the control group. Real time quantitative PCR analysis for ACE, renin and angiotensinogen gene expressions were carried out using a LightCycler™ instrument.
The mean expression ratios were 0.0029 ± 0.0015 for renin, 0.153 ± 0.166 for angiotensinogen, and 0.220 ± 0.294 for ACE, in control samples. The mean expression ratios of pre-eclamptic patients were 0.0061 ± 0.00068, 0.035 ± 0.008, and 0.030 ± 0.006 for renin, angiotensinogen and ACE genes, respectively. While renin expressions increased in the local cord blood of pre-eclampsia in comparison to the normal cord blood, unpredictable decrements in the angiotensinogen and ACE expressions were observed within the same pre-eclamptic samples. There were no statistically significant differences between intrauterine growth restriction (IUGR) and appropriate for gestational age (AGA) newborns in respect to renin, angiotensinogen and ACE gene expressions.
These findings indicate that the gene expression in the major components of the local RAS does not represent a constant mathematical model, but is affected from the ongoing pathobiological events associated with the disease course. Local umbilical cord blood RAS alterations at the basis of genetic expression are evident in pre-eclampsia.
局部自分泌-旁分泌肾素-血管紧张素系统（RAS）独立于循环 RAS 存在于女性生殖系统的主要器官中。我们之前通过验证相应的 ACE、肾素和血管紧张素原 mRNA 证明了“人脐带中的局部 RAS”的存在。本研究旨在探讨子痫前期局部脐带 RAS 的变化。
从 19 例子痫前期患者（年龄 26.6 ± 5.83 岁，范围 18-42 岁）和 20 例正常妊娠妇女（年龄 28.26 ± 7.30 岁，范围 19-37 岁）的脐带血中采集样本。无并发症妊娠的妇女为对照组。使用 LightCyclerTM 仪器对 ACE、肾素和血管紧张素原基因表达进行实时定量 PCR 分析。
对照组样本的肾素表达比值平均值为 0.0029 ± 0.0015，血管紧张素原表达比值平均值为 0.153 ± 0.166，ACE 表达比值平均值为 0.220 ± 0.294。子痫前期患者的肾素表达比值平均值为 0.0061 ± 0.00068，血管紧张素原表达比值平均值为 0.035 ± 0.008，ACE 表达比值平均值为 0.030 ± 0.006。与正常脐带血相比，子痫前期患者脐带血中的肾素表达增加，而同一子痫前期患者的血管紧张素原和 ACE 表达则不可预测地降低。宫内生长受限（IUGR）和适于胎龄（AGA）新生儿之间的肾素、血管紧张素原和 ACE 基因表达无统计学差异。
这些发现表明，局部 RAS 主要成分的基因表达不是一个恒定的数学模型，而是受到与疾病过程相关的持续病理生物学事件的影响。子痫前期时局部脐带血 RAS 改变与遗传表达有关。
