Koca Ebru, Haznedaroglu Ibrahim C, Acar Kadir, Beyazit Yavuz, Aksu Salih, Misirlioglu Muge, Tuncer Serdar, Sayinalp Nilgun, Ozcebe Osman I, Uner Aysegul
Hacettepe University Medical School, Departments of Internal Medicine and Haematology, Ankara, Turkey.
J Renin Angiotensin Aldosterone Syst. 2007 Sep;8(3):145-7. doi: 10.3317/jraas.2007.019.
Local renin-angiotensin system (RAS) may affect leukaemic cell production within the bone marrow microenvironment. Angiotensin-converting enzyme (ACE), renin, and angiotensin could influence leukaemogenesis. In this study, mRNA expressions of the major RAS components (ACE, renin, and angiotensinogen) in K562 human erythroleukaemia cell line have been searched by Real Time quantitative polymerase chain reaction. K562 blasts are multipotential, haematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. We observed significant expressions of ACE, renin, and angiotensinogen in K562 leukaemic blast cells. Therefore, K562 human erythroleukaemia cell line may serve as an in vitro model to elucidate the role of RAS in leukaemia and to test the effects of RAS-affecting drugs on leukaemic cellular proliferation.
局部肾素-血管紧张素系统（RAS）可能影响骨髓微环境中的白血病细胞生成。血管紧张素转换酶（ACE）、肾素和血管紧张素可能影响白血病的发生。在本研究中，通过实时定量聚合酶链反应检测了K562人红白血病细胞系中主要RAS成分（ACE、肾素和血管紧张素原）的mRNA表达。K562母细胞是多能造血恶性细胞，可自发分化为红细胞、粒细胞和单核细胞系列的可识别祖细胞。我们观察到K562白血病母细胞中ACE、肾素和血管紧张素原的显著表达。因此，K562人红白血病细胞系可作为体外模型，以阐明RAS在白血病中的作用，并测试影响RAS的药物对白血病细胞增殖的影响。
