Goharkhay Nina, Lu Jing, Felix Juan C, Wing Deborah A
Department of Obstetrics and Gynecology, University of Texas Medical Branch Galveston, Galveston, TX 77555-0587, USA.
Am J Perinatol. 2007 Sep;24(8):497-500. doi: 10.1055/s-2007-986684. Epub 2007 Sep 12.
Our objective was to assess relative expression levels of mRNA for calcitonin gene-related peptide-receptor component protein (CGRP-RCP) in human myometrium in various physiological states. Using semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), we analyzed myometrial samples from 46 women (10 menopausal, 10 nongravid premenopausal, 19 gravidae, and 7 premenopausal misoprostol-treated nongravid women) for the specific expression of CGRP-RCP mRNA. The expression of CGRP-RCP was significantly increased in gravid compared with nongravid myometrium ( P < 0.002). No significant differences in CGRP-RCP expression were found among the other study groups. We concluded that the increased mRNA expression CGRP-RCP in gravid myometrium supports the possibility of involvement of CGRP in the control of myometrial contractility. Additional studies are necessary to evaluate the exact mechanism of action of CGRP and CGRP-RCP in human myometrium.
我们的目的是评估降钙素基因相关肽受体成分蛋白（CGRP-RCP）的mRNA在不同生理状态下人体子宫肌层中的相对表达水平。我们采用半定量逆转录聚合酶链反应（RT-PCR），分析了46名女性（10名绝经后女性、10名非妊娠绝经前女性、19名孕妇和7名绝经前使用米索前列醇治疗的非妊娠女性）子宫肌层样本中CGRP-RCP mRNA的特异性表达。与非妊娠子宫肌层相比，妊娠子宫肌层中CGRP-RCP的表达显著增加（P<0.002）。在其他研究组之间未发现CGRP-RCP表达有显著差异。我们得出结论，妊娠子宫肌层中CGRP-RCP的mRNA表达增加，支持了CGRP参与子宫肌层收缩控制的可能性。需要进一步研究以评估CGRP和CGRP-RCP在人体子宫肌层中的确切作用机制。
