de la Piedra Concepción, Vicario Carlos, de Acuña Lucrecia Rodríguez, García-Moreno Carmen, Traba Maria Luisa, Arlandis Santiago, Marco Fernando, López-Durán Luis
Fundación Jiménez Díaz, Laboratorio de Fisiopatología Osea, Avenida Reyes Católicos 2, 28040 Madrid, Spain.
J Orthop Res. 2008 Feb;26(2):200-7. doi: 10.1002/jor.20466.
Incorporation of a human bone allograft requires osteoclast activity and growth of recipient osteoblasts. The aim of this work was to study the effects produced by autoclavated and -80 degrees C frozen bone allografts on osteoblast proliferation and synthesis of interleukin 6 (IL6), activator of bone resorption, aminoterminal propeptide of procollagen I (PINP), marker of bone matrix formation, and osteoprotegerin (OPG), inhibitor of osteoclast activity and differentiation. Allografts were obtained from human femoral heads. Human osteoblasts were cultured in the presence (problem group) or in the absence (control group) of allografts during 15 days. Allografts produced a decrease in osteoblast proliferation in the first week of the experiment, and an increase in IL6 mRNA, both at 3 h and 2 days, and an increase in the IL6 released to the culture medium the second day of the experiment. We found a decrease in OPG released to the culture on the 2nd and fourth days. These results suggest an increase in bone resorption and a decrease in bone formation in the first week of the experiment. In the second week, allografts produced an increase in osteoblast proliferation and PINP release to the culture medium, indicating an increase in bone formation; an increase in OPG released to the culture medium, which would indicate a decrease in bone resorption; and a decrease in IL6, indicating a decrease in bone resorption stimulation. These results demonstrate that autoclavated and -80 degrees C frozen bone allografts produce in bone environment changes that regulate their own incorporation to the recipient bone.
人骨同种异体移植的融合需要破骨细胞活性和受体成骨细胞的生长。这项工作的目的是研究经高压灭菌和在 -80℃冷冻的骨同种异体移植对成骨细胞增殖以及白细胞介素6（IL6，骨吸收激活剂）、I型前胶原氨基端前肽（PINP，骨基质形成标志物）和骨保护素（OPG，破骨细胞活性和分化抑制剂）合成的影响。同种异体移植骨取自人股骨头。人成骨细胞在有（实验组）或无（对照组）同种异体移植骨的情况下培养15天。在实验的第一周，同种异体移植骨使成骨细胞增殖减少，在3小时和2天时IL6 mRNA增加，且在实验第二天释放到培养基中的IL6增加。我们发现，在第2天和第4天释放到培养基中的OPG减少。这些结果表明在实验的第一周骨吸收增加而骨形成减少。在第二周，同种异体移植骨使成骨细胞增殖增加以及PINP释放到培养基中增加，表明骨形成增加；释放到培养基中的OPG增加，这表明骨吸收减少；IL6减少，表明骨吸收刺激减少。这些结果表明，经高压灭菌和在 -80℃冷冻的骨同种异体移植在骨环境中产生了调节其自身融入受体骨的变化。
