Wei Yiyong, Hu Yunyu, Hao Wei, Han Yisheng, Meng Guolin, Zhang Dezhi, Wu Zixiang, Wang Haiqiang
Institute of Orthopaedics and Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, People's Republic of China.
J Orthop Res. 2008 Jan;26(1):27-33. doi: 10.1002/jor.20468.
Articular cartilage has a limited self-regenerative capacity. Thus, treatment of cartilage lesions is a major challenge. Tissue engineering using a variety of biomaterials is a promising solution to the problem of cartilage damage. In this in vitro study, we investigated the effect of the presence of cartilage-tissue chondroitin-sulfate (CS) in a fibrin scaffold on the differentiation of adipose-derived adult stem cells (ADAS cells) into chondrocytes. Isolated rabbit ADAS cells were cultured in fibrin matrices with and without CS for up to 14 days. ADAS cells differentiated into chondrocytes in both matrices, but cell proliferation, glycoaminoglycans content, and type II collagen expression were significantly higher in the fibrin-CS matrices than those in the fibrin matrices alone. Histological examination and scanning electronic microscopy revealed the fibrin-CS matrices exceeded in inducing differentiation of ADAS cells into chondrocytes in terms of tissue morphological characteristics. We concluded that the fibrin-CS matrices mimicking native cartilage extracellular matrix could act as a three-dimensional scaffold for cartilage tissue engineering and have the potential for promoting ADAS cells differentiation into chondrocytes.
关节软骨的自我再生能力有限。因此，软骨损伤的治疗是一项重大挑战。使用多种生物材料的组织工程是解决软骨损伤问题的一个有前景的方法。在这项体外研究中，我们研究了纤维蛋白支架中软骨组织硫酸软骨素（CS）的存在对脂肪来源的成体干细胞（ADAS细胞）向软骨细胞分化的影响。将分离的兔ADAS细胞在含CS和不含CS的纤维蛋白基质中培养长达14天。ADAS细胞在两种基质中均分化为软骨细胞，但纤维蛋白-CS基质中的细胞增殖、糖胺聚糖含量和II型胶原表达明显高于单独的纤维蛋白基质。组织学检查和扫描电子显微镜显示，就组织形态特征而言，纤维蛋白-CS基质在诱导ADAS细胞向软骨细胞分化方面更具优势。我们得出结论，模仿天然软骨细胞外基质的纤维蛋白-CS基质可作为软骨组织工程的三维支架，并具有促进ADAS细胞向软骨细胞分化的潜力。
