Puskas Laslo, Puskas Nela, Malobabić Slobodan, Krivokuća Dragan, Stanković Gordana, Radonjić Vidosava
Medicinski fakultet, Institut za anatomiju, Beograd.
Med Pregl. 2007 Jan-Feb;60(1-2):19-24. doi: 10.2298/mpns0702019p.
Morphological features and morphometric parameters of galanin (GAL) and vasoactive intestinal peptide (VIP) immunoreactive neurons and neuronal fibres were studied in all nuclei of adult male rat amygdala.
After perfusion and fixation, rat brains were immunohistochemically stained with antibodies against GAL and VIP and then visualized by avidin-biotin-peroxidase complex.
The greatest number of galanin-immunoreactive neurons were identified in the medial part of the central nucleus and in the dorsal part of the medial nucleus. In the first case, most neurons were bipolar (37%), and in the second, they were ovoid (45%). GAL-immunoreactive fibers were identified in the medial nucleus, "bed nucleus" of the accessory olfactory tract, fiontal cortical nucleus, amygdalo-hippocampal area and basolateral nucleus. VIP-immunoreactive neurons were diffusely distributed in more nuclei than the previous, mostly in the lateral, basolateral, and basomedial nucleus. They were mostly ovoid (40%). VIP-immunoreactive fibers were observed in the lateral part oJ'the central nucleus, while long and radially oriented fibers were present in the frontal and dorsal cortical nucleus.
By distribution analysis of GAL and VIP immunoreactive neurons and fibers, and according to literature data, it can be assumed that the medial part of the central nucleus receives VIP fibers from other parts of the amygdaloid body, and then sends GAL fibers to the medial nucleus.
研究成年雄性大鼠杏仁核所有核团中甘丙肽（GAL）和血管活性肠肽（VIP）免疫反应性神经元及神经纤维的形态学特征和形态计量学参数。
灌注固定后，用抗GAL和VIP的抗体对大鼠脑进行免疫组织化学染色，然后用抗生物素蛋白-生物素-过氧化物酶复合物进行可视化处理。
在中央核内侧部分和内侧核背侧部分发现数量最多的甘丙肽免疫反应性神经元。在第一种情况下，大多数神经元为双极神经元（37%），在第二种情况下，它们为卵圆形（45%）。在内侧核、副嗅束“终纹床核”、额叶皮质核、杏仁体-海马区和基底外侧核中发现了GAL免疫反应性纤维。VIP免疫反应性神经元比前者更广泛地分布于更多核团中，主要分布在外侧核、基底外侧核和基底内侧核。它们大多为卵圆形（40%）。在中央核外侧部分观察到VIP免疫反应性纤维，而在额叶和背侧皮质核中存在长的且呈放射状排列的纤维。
通过对GAL和VIP免疫反应性神经元及纤维的分布分析，并根据文献数据，可以推测中央核内侧部分从杏仁体其他部分接收VIP纤维，然后向内侧核发送GAL纤维。
