Hoda Rana S
Department of Pathology, Cytopathology Unit, University of Rochester, Rochester, New York, USA.
Diagn Cytopathol. 2007 Oct;35(10):621-34. doi: 10.1002/dc.20698.
Liquid-based preparations (LBP) are increasingly being used both for gynecologic (gyn) and non-gynecologic (non-gyn) cytology including fine needle aspirations (FNA). The two FDA-approved LBP currently in use include ThinPrep (TP), (Cytyc Corp, Marlborough, MA) and SurePath (SP), (TriPath Imaging Inc., Burlington, NC). TP was approved for cervico-vaginal (Pap test) cytology in 1996 and SP in 1999 and both have since also been used for non-gyn cytology. In the LBP, instead of being smeared, cells are rinsed into a liquid preservative collection medium and processed on automated devices. Even after a decade of use, the morphological interpretation of LBP remains a diagnostic challenge because of somewhat altered morphology and artifacts or facts resulting from the fixation and processing techniques. These changes include cleaner background with altered or reduced background and extracellular elements; architectural changes such as smaller cell clusters and sheets, breakage of papillae; altered cell distribution with more dyscohesion and changes in cellular morphology with enhanced nuclear features, smaller cell size and slightly more three-dimensional (3-D) clusters. Herein, we review the published literature on morphological aspects of LBP for non-gyn cytology.
液基制剂（LBP）越来越多地用于妇科（gyn）和非妇科（non-gyn）细胞学检查，包括细针穿刺抽吸（FNA）。目前美国食品药品监督管理局（FDA）批准使用的两种LBP包括ThinPrep（TP，Cytyc公司，马萨诸塞州马尔伯勒）和SurePath（SP，TriPath影像公司，北卡罗来纳州伯灵顿）。TP于1996年被批准用于宫颈阴道（巴氏试验）细胞学检查，SP于1999年被批准，此后两者也都用于非妇科细胞学检查。在LBP中，细胞不是被涂片，而是被冲洗到液体保存收集介质中，并在自动化设备上进行处理。即使经过十年的使用，由于固定和处理技术导致的形态改变和假象或事实，LBP的形态学解读仍然是一个诊断挑战。这些变化包括背景更清晰，背景和细胞外成分改变或减少；结构变化，如细胞团簇和细胞片更小、乳头破裂；细胞分布改变，细胞间粘附性增加，细胞形态改变，核特征增强，细胞尺寸更小，三维（3-D）团簇略多。在此，我们回顾了已发表的关于非妇科细胞学LBP形态学方面的文献。
