Pinto Alvaro P, Maia Henrique Felde, di Loretto Celso, Krunn Patrícia, Túlio Siumara, Collaço Luis Martins
Department of Pathology, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
Diagn Cytopathol. 2007 Oct;35(10):663-9. doi: 10.1002/dc.20654.
This study investigates the rule that repeating cytological preparations on liquid-based cytology improves sample adequacy, diagnosis, microbiological, and hormonal evaluations. We reviewed 156 cases of pap-stained preparations of exfoliated cervical cells in two slides processed by DNA-Cytoliq System. After sample repeat/dilution, limiting factors affecting sample adequacy were removed in nine cases and three unsatisfactory cases were reclassified as satisfactory. Diagnosis was altered in 24 cases. Of these, the original diagnosis in 15 was atypical squamous cells of undetermined significance; after the second slide examination, diagnosis in 5 of the 15 cases changed to low-grade squamous intraepithelial lesion, 3 to high-grade squamous intraepithelial lesion, and 7 to absence of lesion. Microbiological evaluation was altered, with Candida sp. detected in two repeated slides. Repeat slide preparation or dilution of residual samples enhances cytological diagnosis and decreases effects of limiting factors in manually processed DIGENE DCS LBC.
本研究调查了在液基细胞学上重复进行细胞学制片可提高样本充足率、诊断、微生物学及激素评估的规律。我们回顾了通过DNA-Cytoliq系统处理的两张载玻片上156例宫颈脱落细胞巴氏染色制片。样本重复/稀释后，9例中影响样本充足率的限制因素被消除，3例不满意病例被重新分类为满意。24例诊断发生改变。其中，原诊断为意义不明确的非典型鳞状细胞的有15例；第二次载玻片检查后，15例中的5例诊断变为低级别鳞状上皮内病变，3例变为高级别鳞状上皮内病变，7例变为无病变。微生物学评估发生改变，在两张重复制片中检测到念珠菌属。重复制片或残留样本稀释可增强细胞学诊断，并减少手工处理的DIGENE DCS LBC中限制因素的影响。
