Hua Dong, Huang Zhao-Hui, Mao Yong, Deng Jian-Zhong
Oncology Institute of Wuxi, The Fourth Affiliated Hospital of Suzhou University, 200 Huihe Road, Wuxi 214062, Jiangsu Province, China.
World J Gastroenterol. 2007 Oct 7;13(37):5030-4. doi: 10.3748/wjg.v13.i37.5030.
To investigate the prognostic role of thymidylate synthase (TS) and thymidine phosphorylase (TP) mRNA levels in T3 or T4 gastric cancer treated with 5-fluorouracil-based adjuvant chemotherapy.
Fifty-one patients with T3 or T4 gastric cancer received systemic 5-fluorouracil-based adjuvant chemotherapy, and intratumoral expression of TS and TP in 51 gastric cancer tissue samples was tested by real-time quantitative PCR.
The median disease-free survival (DFS) time was 10.2 mo in the patients. There were no significant differences in DFS between the groups with high and low levels of TP. However, the group with low level of TS had a longer DFS (14.4 mo vs 8.3 mo, P = 0.017). The median overall survival (OS) time was 18.5 mo, and there were significant differences in OS between the groups with high and low levels of TS or TP (for TS, 17.0 mo vs 21.3 mo, P = 0.010; for TP, 16.6 mo vs 22.5 mo, P = 0.009). Moreover, the coupled low expression of these two genes was strongly associated with a longer survival time of patients as compared with that of a single gene.
Expression of TS and TP mRNA is a useful predictive parameter for the survival of postoperative gastric cancer patients after 5-fluorouracil-based adjuvant chemotherapy.
探讨胸苷酸合成酶（TS）和胸苷磷酸化酶（TP）mRNA水平在接受以5-氟尿嘧啶为基础的辅助化疗的T3或T4期胃癌中的预后作用。
51例T3或T4期胃癌患者接受了以5-氟尿嘧啶为基础的全身辅助化疗，采用实时定量PCR检测51份胃癌组织样本中TS和TP的瘤内表达。
患者的无病生存期（DFS）中位数为10.2个月。TP水平高低组之间的DFS无显著差异。然而，TS水平低的组DFS更长（14.4个月对8.3个月，P = 0.017）。总生存期（OS）中位数为18.5个月，TS或TP水平高低组之间的OS有显著差异（TS：17.0个月对21.3个月，P = 0.010；TP：16.6个月对22.5个月，P = 0.009）。此外，与单个基因低表达相比，这两个基因的联合低表达与患者更长的生存时间密切相关。
TS和TP mRNA的表达是接受以5-氟尿嘧啶为基础的辅助化疗的胃癌术后患者生存的有用预测参数。
