DU Yong-ping, Zhang Yue-ping, Zhang Guo-cheng
Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province 710032, China.
Zhong Xi Yi Jie He Xue Bao. 2007 Sep;5(5):550-4. doi: 10.3736/jcim20070515.
To investigate the effects of Baobaole oral liquid on neuronal excitability in lateral hypothalamic area (LHA) and ventromedial hypothalamic nuclear (VMN) in anorectic rats.
The anorectic rat model was established by feeding with special prepared forage for a week, and then Baobaole oral liquid, a liquid extract of a compound traditional Chinese medicine for activating spleen, was administered once a day for 3 weeks. Finally, extracellular recording from LHA and VMN neurons in rats were made in order to characterize their responses to gastric vagal nerve stimulation and intravenous injection of glucose in the normal, untreated, and Baobaole-treated groups.
There was no statistical difference in response characteristics of LHA neurons to gastric vagal stimulation among 3 groups. The duration of VMN neuron excitation response to gastric vagal nerve stimulation in the untreated group was significantly longer than that of the normal control group (P<0.01), while the required stimulation intensity was significantly decreased (P<0.01). Moreover, among the neurons responding to the gastric vagal stimulation in the untreated group, the number of glycemia-sensitive neurons decreased in LHA and increased in VMN (P<0.01). The gastric vagal stimulation induced neuron responses in LHA and VMN of the Baobaole-treated group were not significantly changed as compared with the normal control group (P<0.01), and neither were the intravenous injection of glucose induced responses.
Baobaole oral liquid can modulate the sensitivity of LHA and VMN neurons to the peripheral signal and make the coordination between LHA and VMN neurons in order to improve the appetite of anorectic rats.
探讨保宝乐口服液对厌食大鼠下丘脑外侧区（LHA）和下丘脑腹内侧核（VMN）神经元兴奋性的影响。
通过喂食特制饲料一周建立厌食大鼠模型，然后给予保宝乐口服液（一种复方健脾中药的液体提取物），每天给药1次，持续3周。最后，对正常组、未处理组和保宝乐处理组大鼠的LHA和VMN神经元进行细胞外记录，以表征它们对胃迷走神经刺激和静脉注射葡萄糖的反应。
3组中LHA神经元对胃迷走神经刺激的反应特性无统计学差异。未处理组VMN神经元对胃迷走神经刺激的兴奋反应持续时间显著长于正常对照组（P<0.01），而所需刺激强度显著降低（P<0.01）。此外，在未处理组中对胃迷走神经刺激有反应的神经元中，LHA中血糖敏感神经元数量减少，VMN中血糖敏感神经元数量增加（P<0.01）。与正常对照组相比，保宝乐处理组大鼠LHA和VMN中胃迷走神经刺激诱导的神经元反应无显著变化（P<0.01），静脉注射葡萄糖诱导的反应也无显著变化。
保宝乐口服液可调节LHA和VMN神经元对外周信号的敏感性，使LHA和VMN神经元之间的协调有序，从而改善厌食大鼠的食欲。
