Nogal Ana, Coelho Ana, Catarino Raquel, Morais António, Lobo Francisco, Medeiros Rui
Molecular Oncology Department, Portuguese Institute of Oncology, Rua Dr. António Bernardino Almeida, 4200-072 Porto, Portugal.
Cancer Genet Cytogenet. 2007 Sep;177(2):149-52. doi: 10.1016/j.cancergencyto.2007.06.011.
Testosterone exposure has been implicated in prostate carcinogenesis, and genes that alter its metabolism, such as CYP3A4, have been associated with prostate cancer susceptibility. The aim of our study was to assess the relationship between the CYP3A4 1B polymorphism and its possible role in the development of prostate cancer. DNA samples obtained from the peripheral blood cells of 414 individuals diagnosed with prostate cancer and 337 healthy male donors were used in this case-control study. The CYP3A41B polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism methodology. We found no statistically significant differences in the distribution of the CYP3A41B genotypes between cases and controls (P = 0.470; odds ratio = 1.191; 95% confidence interval=0.740-1.918), as well as after the stratification of our analysis, according to important clinicopathologic parameters of prostate cancer. Our results suggest that the CYP3A41B polymorphism is not associated with prostate cancer risk within the Portuguese population.
睾酮暴露与前列腺癌发生有关，而改变其代谢的基因，如CYP3A4，已与前列腺癌易感性相关。我们研究的目的是评估CYP3A4 1B多态性与前列腺癌发生发展的可能关系。在这项病例对照研究中，使用了从414名被诊断为前列腺癌的个体和337名健康男性献血者的外周血细胞中获取的DNA样本。采用聚合酶链反应-限制性片段长度多态性方法分析CYP3A41B多态性。我们发现病例组和对照组之间CYP3A41B基因型的分布没有统计学显著差异（P = 0.470；优势比 = 1.191；95%置信区间 = 0.740 - 1.918），并且在根据前列腺癌重要临床病理参数进行分层分析后也是如此。我们的结果表明，在葡萄牙人群中，CYP3A41B多态性与前列腺癌风险无关。
