Wu Sheng-Long, Bao Wen-Bin, Ju Hui-Ping, Zhu Guo-Qiang, Li Bi-Chun, Chen Guo-Hong
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009 China.
Yi Chuan. 2007 Sep;29(9):1071-6. doi: 10.1360/yc-007-1071.
Enterotoxigenic Escherichia coli F18 (ETEC F18) is the main pathogen that causes edema disease and post-weaning diarrhea in piglets, and a1-fucosytransferase (FUT1) gene has been identified as a receptor gene encoding the receptor for ETEC F18 bacteria. In this study, the method of PCR-RFLP was used to investigate the among 21 breeds including one wild boar breed and 20 western commercial and Chinese native pig breeds (populations). The results showed that none of the individuals in all 21 breeds possessed the resistant AA genotype, the genetic polymorphisms of the FUT1 locus were only detected in two western pig breeds (Duroc and Yorkshire), Lingao pig and hybrid pig breeds, while the wild boar and all the other Chinese pig breeds only possessed the susceptible GG genotype. The results indicated that Chinese native pig breeds, unlike western pig breeds, lack the genetic background on the resistance to ETEC F18 bacteria. This may be owe to their different origination, as the resistance gene to ETEC F18 might be originated from European wild boar. It was also inferred that edema disease and post-weaning diarrhea caused by ETEC F18 had close relationship with the growth speed of pigs.
产肠毒素大肠杆菌F18（ETEC F18）是引起仔猪水肿病和断奶后腹泻的主要病原菌，α1-岩藻糖基转移酶（FUT1）基因已被鉴定为编码ETEC F18细菌受体的受体基因。本研究采用PCR-RFLP方法对包括1个野猪品种和20个西方商业猪品种及中国地方猪品种（群体）在内的21个品种进行了调查。结果表明，21个品种的所有个体均不具有抗性AA基因型，FUT1位点的遗传多态性仅在两个西方猪品种（杜洛克和约克夏）、临高猪和杂交猪品种中检测到，而野猪和所有其他中国猪品种仅具有易感GG基因型。结果表明，与西方猪品种不同，中国地方猪品种缺乏对ETEC F18细菌抗性的遗传背景。这可能归因于它们不同的起源，因为对ETEC F18的抗性基因可能起源于欧洲野猪。还推断出由ETEC F18引起的水肿病和断奶后腹泻与猪的生长速度密切相关。
