Xu Chuan-Mei, Bie Tong-De, Wang Chun-Mei, Zhou Bo, Chen Pei-Du
1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. xuchuanmei1979 @126.com
Yi Chuan. 2007 Sep;29(9):1126-30. doi: 10.1360/yc-007-1126.
Fluorescent in situ hybridization (FISH) and sequential C-banding and FISH techniques were used in locating 45S rDNA sequence on specific chromosomes of Triticum monococcum, T. dicoccoides, T. aestivum, Hordeum vulgare, Haynaldia villosa, T. durum-Haynaldia villosa amphiploid, Avena fatus, and Roegneria kamoji. High polymorphism between common wheat and its relatives on 45S rDNA sequence distribution was detected. All the chromosomal secondary constriction regions of these species had 45S rDNA loci. Some non-satellite chromosomes had additional 45S rDNA loci. A 45S rDNA locus was located on the terminal region of the short arm of R. kamoji chromosome 1Rk#1.
采用荧光原位杂交（FISH）以及顺序C分带和FISH技术，在一粒小麦、野生二粒小麦、普通小麦、大麦、簇毛麦、硬粒小麦-簇毛麦双二倍体、燕麦和鹅观草的特定染色体上定位45S rDNA序列。检测到普通小麦与其近缘种在45S rDNA序列分布上存在高度多态性。这些物种所有的染色体次缢痕区域都有45S rDNA位点。一些非随体染色体有额外的45S rDNA位点。一个45S rDNA位点位于鹅观草1Rk#1染色体短臂的末端区域。
