[转OsMAPK4基因烟草的抗旱性及遗传特性]

[Drought resistance and heredity of transgenic tobacco with OsMAPK4 gene].

作者信息

Li Jie, Qi Yan, Li Ying, Liu Xi-Yan, Ji Haobutao, Zhu Yan-Ming, Bai Xi, Cai Hua

机构信息

Life Science College, Northeast Agricultural University, Harbin 150030, China E-mail.

出版信息

Yi Chuan. 2007 Sep;29(9):1144-8. doi: 10.1360/yc-007-1144.

DOI:10.1360/yc-007-1144

PMID:17855267

Abstract

OsMAPK4 gene was obtained by RT-PCR using gene special primer from Liaoyan241 leaf treated with low temperature. Plant expression vector pBME12 was constructed in which OsMAPK4 gene was regulated by E12 promoter. OsMAPK4 gene was transferred into tobacco via Agrobacterium-mediated method and 25 transgenic plants obtained. The results of drought resistance experiments showed that overexpression of OsMAPK4 gene conferred enhanced drought resistance of T1 transgenic plants. Kanamycin-resistance segregation in T1 transgenic plants showed that the most transgenic lines were in single gene inheritance.

摘要

使用基因特异性引物通过RT-PCR从经低温处理的辽烟241叶片中获得OsMAPK4基因。构建了植物表达载体pBME12，其中OsMAPK4基因由E12启动子调控。通过农杆菌介导的方法将OsMAPK4基因转入烟草，获得了25株转基因植株。抗旱性实验结果表明，OsMAPK4基因的过表达赋予了T1代转基因植株增强的抗旱性。T1代转基因植株中的卡那霉素抗性分离表明，大多数转基因株系呈单基因遗传。