[Soybean - improves drought resistance of transgenic tobacco].

Galactinol synthase (GolS) genes play important roles in plant response to abiotic stress. In this research, the plant expression vector of soybean - gene was constructed and transformed into tobacco to study the drought tolerance of transgenic tobacco. A - gene with 975 bp coding sequence was cloned from soybean leaves by reverse transcription-polymerase chain reaction (RT-PCR). - was linked to the plant expression vector pRI101 by restriction enzyme sites Ⅰ and R Ⅰ, and transformed into tobacco by leaf disc method. Genomic DNA PCR and real-time PCR showed that three - transgenic tobacco plants were obtained. The growth status of - transgenic tobacco under drought stress was better than that of wild-type tobacco. After drought stress treatment, the electrolyte leakage and malondialdehyde content of transgenic tobacco were lower than those of wild-type tobacco, but the proline content and soluble sugar content were higher than those of wild-type tobacco. The results of real-time PCR showed that the heterologous expression of - increased the expression of stress-related genes and in transgenic tobacco. The above results indicated that - improved drought resistance of transgenic tobacco.