Wakitani S, Nawata M, Kawaguchi A, Okabe T, Takaoka K, Tsuchiya T, Nakaoka R, Masuda H, Miyazaki K
Department of Orthopaedic Surgery, Osaka City University Graduate School of Medicine, 1-4-3 Asahi, Abeno-ku, Osaka 545-8585, Japan.
Rheumatology (Oxford). 2007 Nov;46(11):1652-6. doi: 10.1093/rheumatology/kem220. Epub 2007 Sep 12.
To find serum markers that may serve as indices for an early diagnosis of degeneration or damage of the articular cartilage.
Twenty-four healthy volunteers, 19 individuals with knee trauma (KT) and 31 with knee osteoarthritis (OA) were evaluated. KT patients were divided into a group (n = 5) with an injury <2 months old (recent KT) and a group (n = 14) with that >2 months old (old KT). Articular cartilage damage was assessed using either arthroscopy or direct observation. Serum concentrations of hyaluronic acid (HA), cartilage proteoglycan aggrecan turnover epitope (CS846) and cartilage oligomeric protein (COMP) were measured using enzyme-linked immunosorbent assay kits and those of keratan sulfate (KS) and chondroitin-6-sulfate (C6S) using high-performance liquid chromatography.
Serum KS in the recent KT group (2095 +/- 594 ng/ml) was significantly higher than that in the old KT group (1373 +/- 418 ng/ml; P = 0.021), and serum COMP in the recent KT group (1572 +/- 182 ng/ml) showed a tendency that was higher than that in the old KT group (1350 +/- 250 ng/ml; P = 0.079). Serum KS in OA patients with Kellgren and Lawrence (KL) grades 0 and I (1456 +/- 334 ng/ml) showed a tendency that was higher than that in OA patients with KL grades II, III and IV (1248 +/- 220 ng/ml; P = 0.084).
The serum concentration of KS correlated with the damage of the articular cartilage and it was significantly increased even at an early stage after the injury.
寻找可作为关节软骨退变或损伤早期诊断指标的血清标志物。
对24名健康志愿者、19名膝关节创伤（KT）患者和31名膝关节骨关节炎（OA）患者进行评估。KT患者分为损伤<2个月的组（n = 5，近期KT）和损伤>2个月的组（n = 14，陈旧KT）。使用关节镜检查或直接观察评估关节软骨损伤情况。采用酶联免疫吸附测定试剂盒测定血清透明质酸（HA）、软骨蛋白聚糖聚集蛋白聚糖周转表位（CS846）和软骨寡聚蛋白（COMP）的浓度，采用高效液相色谱法测定硫酸角质素（KS）和硫酸软骨素-6-硫酸酯（C6S）的浓度。
近期KT组血清KS（2095±594 ng/ml）显著高于陈旧KT组（1373±418 ng/ml；P = 0.021），近期KT组血清COMP（1572±182 ng/ml）有高于陈旧KT组的趋势（1350±250 ng/ml；P = 0.079）。Kellgren和Lawrence（KL）分级为0级和I级的OA患者血清KS（1456±334 ng/ml）有高于KL分级为II级、III级和IV级的OA患者的趋势（1248±220 ng/ml；P = 0.084）。
血清KS浓度与关节软骨损伤相关，且在损伤后早期即显著升高。
