Li Longkun, Jiang Chonghe, Hao Ping, Li Weibing, Song Caiping, Song Bo
Center of Urology, Southwest Hospital, Third Military Medical University, Chongqing, China.
Am J Physiol Cell Physiol. 2007 Nov;293(5):C1627-35. doi: 10.1152/ajpcell.00122.2007. Epub 2007 Sep 13.
To evaluate the changes in intercellular communication through gap junctions in detrusor overactivity (DO), we studied 23 adult female Wistar rats with DO after partial outflow obstruction (DO group) and 13 sham-operated rats (control group). The two groups were compared by means of urodynamics, light and electron microscopy, expression of Cx40, Cx43, and Cx45 mRNA genes with RT-PCR, Cx43 protein with Western blot analysis, and functional intercellular communication with scrape loading dye transfer (SLDT) and fluorescence recovery after photobleaching (FRAP). The number of gap junctions and the expression of connexin mRNA and Cx43 protein were increased in DO rats, and intercellular communication through gap junctions increased after 6 wk of partial outflow obstruction as assessed with SLDT and FRAP techniques. The findings provide a theoretical rationale for using Cx43 antagonists and gap junction inhibitors in the treatment of patients with overactive detrusor secondary to partial bladder outflow obstruction.
为评估逼尿肌过度活动（DO）时通过缝隙连接的细胞间通讯变化，我们研究了23只成年雌性Wistar大鼠，这些大鼠在部分流出道梗阻后出现DO（DO组）以及13只假手术大鼠（对照组）。通过尿动力学、光镜和电镜、采用逆转录聚合酶链反应（RT-PCR）检测Cx40、Cx43和Cx45 mRNA基因的表达、采用蛋白质印迹分析检测Cx43蛋白以及采用刮擦负载染料转移（SLDT）和光漂白后荧光恢复（FRAP）检测功能性细胞间通讯，对两组进行比较。DO大鼠的缝隙连接数量以及连接蛋白mRNA和Cx43蛋白的表达增加，并且采用SLDT和FRAP技术评估发现，部分流出道梗阻6周后通过缝隙连接的细胞间通讯增加。这些发现为使用Cx43拮抗剂和缝隙连接抑制剂治疗继发于部分膀胱流出道梗阻的逼尿肌过度活动患者提供了理论依据。
