Takizawa S
Gan. 1976 Feb;67(1):33-40.
A total 39 kidney tumors were induced by a continuous oral administration of N-butylnitrosourea (BNU) in 33 out of 204 rats of W/Fu strain and of 41 (W/Fu X ACI/N)F1 rats. No spontaneous renal tumors were observed among 66 males and 109 females of W/Fu rats which survived beyond the age of 19 months. Histologically, renal cell and mesenchymal types were commonly observed; 24 cases belonging to the former and 11 cases to the latter. Two cases of nephroblastoma were also encountered. There was no sex difference in renal tumorigenesis with BNU as a whole. Castration in both sexes was apparently inhibitory for kidney tumor development. Estrogenization of castrated rats either by syngeneic ovary graft or by repeated injections of estradiol benzoate enhanced tumor induction with BNU. Progesterone was not effective in restoring the tumor incidence in castrated rats. Distribution of histological types of tumors thus induced differed among the hosts with different hormonal conditions; in males the majority was renal cell type, whereas almost all mesenchymal tumor and nephroblastoma cases were found in intact females or estrogenized rats. BNU induced a variety of tumors in several organs including cerebral hemisphere, peripheral nerves, mammary glands, hematopoietic system, digestive tracts, and so on. However, such concurrence did not affect the development of renal tumors in the present study.
通过对204只W/Fu品系大鼠中的33只以及41只（W/Fu×ACI/N）F1大鼠连续口服N-丁基亚硝脲（BNU），共诱发了39个肾肿瘤。在存活超过19个月的66只雄性和109只雌性W/Fu大鼠中未观察到自发性肾肿瘤。组织学上，常见肾细胞型和间充质型；前者有24例，后者有11例。还发现了2例肾母细胞瘤。总体而言，BNU诱发肾肿瘤没有性别差异。对两性进行去势明显抑制肾肿瘤的发展。通过同种卵巢移植或反复注射苯甲酸雌二醇使去势大鼠雌激素化，可增强BNU诱发肿瘤的能力。孕酮对恢复去势大鼠的肿瘤发生率无效。在不同激素状态的宿主中，如此诱发的肿瘤组织学类型分布不同；在雄性中，大多数是肾细胞型，而几乎所有间充质肿瘤和肾母细胞瘤病例都见于未阉割的雌性或雌激素化的大鼠。BNU在包括大脑半球、外周神经、乳腺、造血系统、消化道等多个器官中诱发了多种肿瘤。然而，在本研究中，这种并发情况并不影响肾肿瘤的发展。
