van Damme H S, Beugeling T, Ratering M T, Feijen J
Department of Chemical Technology, University of Twente, Enschede, The Netherlands.
J Biomater Sci Polym Ed. 1991;3(1):69-84. doi: 10.1163/156856292x00088.
Protein adsorption of human serum albumin (HSA), human fibrinogen (Fg), human immunoglobulin G (IgG), high density lipoprotein (HDL) and high molecular weight kininogen (HMWK) from plasma onto poly (n-alkyl methacrylate) (PAMA) surfaces was measured using a semi-quantitative enzyme-immunoassay. Adsorption was investigated for PAMA(n = 1) (n is the number of C-atoms in the n-alkyl side chain), PAMA(n = 8) and PAMA(n = 18). PAMA(n = 1) has a relatively hydrophilic surface as compared to the more hydrophobic PAMA(n = 8) surface. Both polymers have surface chains which do not reorient after contact with water. The PAMA(n = 18) surface is relatively hydrophobic but in this case polymer surface chains and segments are able to reorient after contact with water. Protein adsorption was measured both as a function of time and as a function of the plasma dilution. If adsorption from plasma was measured as a function of time no exchange of proteins could be observed. The amount of adsorbed protein was always larger in the case of the hydrophobic PAMA(n = 8) as compared to PAMAS(n = 1 and 18), probably due to hydrophobic interactions between the proteins and the PAMA(n = 8) surface. At high plasma concentration relatively large amounts of HDL adsorb onto PAMA(n = 8), indicating that this lipoprotein preferentially adsorbs onto this surface.
使用半定量酶免疫测定法测量了血浆中的人血清白蛋白（HSA）、人纤维蛋白原（Fg）、人免疫球蛋白G（IgG）、高密度脂蛋白（HDL）和高分子量激肽原（HMWK）在聚（甲基丙烯酸正烷基酯）（PAMA）表面的蛋白质吸附情况。研究了PAMA（n = 1）（n为正烷基侧链中C原子的数量）、PAMA（n = 8）和PAMA（n = 18）的吸附情况。与疏水性更强的PAMA（n = 8）表面相比，PAMA（n = 1）具有相对亲水性的表面。两种聚合物的表面链在与水接触后都不会重新取向。PAMA（n = 18）表面相对疏水，但在这种情况下，聚合物表面链和链段在与水接触后能够重新取向。测量了蛋白质吸附量随时间和血浆稀释度的变化情况。如果测量血浆中的吸附量随时间的变化，未观察到蛋白质的交换。与PAMA（n = 1和18）相比，疏水性的PAMA（n = 8）表面吸附的蛋白质量总是更大，这可能是由于蛋白质与PAMA（n = 8）表面之间的疏水相互作用。在高血浆浓度下，相对大量的HDL吸附到PAMA（n = 8）上，表明这种脂蛋白优先吸附到该表面。
